Tris-Borate-Edta Buffer

Tris-Borate-Edta Buffer

Synonyms TBE buffer

CAS Number: Not applicable

MDL Number: MFCD00236358

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SKU 04816721-CF
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$56.35
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Description

Product Description

Tris-Borate-Edta Buffer

Application Notes

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. It is used to prepare a 1 X buffer for polyacrylamide and agarose gel electrophoresis. When diluted to a 1X concentration, it yields a TBE buffer solution that contains 100 mM Tris, 90 mM boric acid and 1mM EDTA. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Buffer for DNA and RNA polyacrylamide gel electrophoresis

Specifications
SKU 04816721-CF
Alternate Names TBE buffer
Application Notes TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. It is used to prepare a 1 X buffer for polyacrylamide and agarose gel electrophoresis. When diluted to a 1X concentration, it yields a TBE buffer solution that contains 100 mM Tris, 90 mM boric acid and 1mM EDTA. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Format solution
Hazard Statements H360FD
Applications Buffer for DNA and RNA polyacrylamide gel electrophoresis
MDL # MFCD00236358
Personal Protective Equipment Eyeshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter
Physical Appearance Clear; Colorless Solution
Product Families Description Tris-Borate-EDTA Buffer
Product Overview Tris-Borate-Edta Buffer
Safety Symbol GHS08
Storage and Handling Store at Room Temperature(15-30 °C).
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.