Tricine
Product Description
Tricine
Application Notes
Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
Usage Statement
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
| SKU | 04807410-CF |
| Alternate Names | N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine |
| Application Notes | Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa. |
| Beilstein Registry Number | 1937804 |
| EC Number | 227-193-6 |
| Format | powder |
| Grade | Electrophoresis Grade |
| Hazard Statements | H317 |
| Heavy Metals | (as Pb) |
| Insoluble Matter | ≤10 ppm |
| Mean Pore Size | MFCD00004277 |
| Molecular Formula | C6H13NO5 |
| Optical Rotation | DpK/DT: -0.021(Lit.); Metal Binding Constants (log K) for 0.1 M solution at 20 °C: Mg2+: 1.2 Ca2+: 2.4 Mn2+: 2.7 Cu2+: 7.3(Lit.) |
| Physical Appearance | White Powder |
| pKa | 4.5 - 5.5 (1% aq soln) ; Buffering pH range 7.4-8.8(Lit.) |
| Potency | 8.1 (25 °C)(Lit.) |
| Product Families Description | Tricine |
| Product Overview | Tricine |
| Purity | ≥99% |
| Source | Very soluble in water (25% w/v - clear; colorless solution). |
| Storage and Handling | Store at Room Temperature (15-30 °C). |
| Sulphated Ash | Cell culture tested |
| Unit Definition | A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH. |
| Usage Statement | Research Use Only (RUO). Ready for CE IVD labeling of clinical applications. |