Dino

Tricine

Tricine

Synonyms N-Tris-[hydroxymethyl]methylglycine; N-[2-Hydroxy-1,1-bis(hydroxymethyl) ethyl] glycine

CAS Number: 5704-04-1 Molecular Formula: C6H13NO5

Beilstein Registry Number: 1937804 EC Number: 227-193-6

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SKU 04807410-CF
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Description

Product Description

Tricine

Application Notes

Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Specifications
SKU 04807410-CF
Application Notes Tricine is used as buffer component for separation of low molecular weight peptides. Tricine can be used in cryopreservation medium for the preservation of tissues and organs. Cryopreservation depends on the physical and chemical characteristics of the preservation medium used. The pH values and pK values for tricine/DMSO mixtures has been reported down to -20 °C. Tricine has been found to be an efficient scavenger of hydroxyl radicals in a study of radiation-induced membrane damage. Tricine is typically the buffer of choice in SDS-PAGE systems when separating proteins in the range of 1 to 100 kDa.
Beilstein Registry Number 1937804
EC Number 227-193-6
Format powder
Grade Electrophoresis Grade
Hazard Statements H317
Heavy Metals (as Pb)
Insoluble Matter ≤10 ppm
Mean Pore Size MFCD00004277
Molecular Formula C6H13NO5
Optical Rotation DpK/DT: -0.021(Lit.); Metal Binding Constants (log K) for 0.1 M solution at 20 °C: Mg2+: 1.2 Ca2+: 2.4 Mn2+: 2.7 Cu2+: 7.3(Lit.)
Physical Appearance White Powder
pKa 4.5 - 5.5 (1% aq soln) ; Buffering pH range 7.4-8.8(Lit.)
Potency 8.1 (25 °C)(Lit.)
Product Families Description Tricine
Product Overview Tricine
Purity ≥99%
Source Very soluble in water (25% w/v - clear; colorless solution).
Storage and Handling Store at Room Temperature (15-30 °C).
Sulphated Ash Cell culture tested
Unit Definition A buffer may be prepared by titrating with sodium hydroxide to the desired pH, using about a half-equivalent of NaOH.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.