Temed

Temed

Synonyms 1,2-Bis(dimethylamino)ethane; N,N,N′,N′-Tetramethylethylenediamine; TMEDA

CAS Number: 110-18-9 Molecular Formula: C6H16N2 Molecular Weight: 116.208 g/mol

Beilstein Registry Number: 1732991 EC Number: 203-744-6 MDL Number: MFCD00008335

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SKU 04805613-CF
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Description

Product Description

Temed

Application Notes

N,N,N',N'-Tetramethylethylenediamine is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules such as nucleic acids and proteins. TEMED has been used in the coating for soft polymers, such as polyacrylamide, in capillaries for investigation by atom force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED. It is also used used in the HPLC of small organic molecules.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Polyacrylamide gel formation catalyst

Specifications
SKU 04805613-CF
Alternate Names 1,2-Bis(dimethylamino)ethane; N,N,N′,N′-Tetramethylethylenediamine; TMEDA
Application Notes N,N,N',N'-Tetramethylethylenediamine is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules such as nucleic acids and proteins. TEMED has been used in the coating for soft polymers, such as polyacrylamide, in capillaries for investigation by atom force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED. It is also used used in the HPLC of small organic molecules.
Beilstein Registry Number 1732991
Boiling Point 250 to 252° F at 760 mm Hg (NTP, 1992)
Density 0.7765 at 68° F (NTP, 1992)
EC Number 203-744-6
Flash Point 50° F (NTP, 1992)
Format liquid
Grade Electrophoresis Grade
Hazard Statements H225-H302-H314-H332
Applications Polyacrylamide gel formation catalyst
MDL # MFCD00008335
Melting Point -67.2° F (NTP, 1992)
Moisture Content ≤2%
Molecular Formula C6H16N2
Molecular Weight 116.208 g/mol
Personal Protective Equipment Faceshields, full-face respirator , Gloves, Goggles, multi-purpose combination respirator cartridge,respirator filter
Physical Appearance Liquid
Product Families Description N,N,N',N'-Tetramethylethylenediamine
Product Overview Temed
Purity ≥99%
Refractive Index n20/D 1.4179 (Lit.)
RTECS Number KV7175000
Safety Symbol GHS02, GHS05, GHS07
Solubility Soluble (NTP, 1992)
Storage and Handling Store at Room Temperature (15-30 °C); store under nitrogen.
Typical Working Concentration Suggested use is 6-10 uL for 10 mL acrylamide gel solution (the less amount of TEMED used, the slower the polymerization)
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.