Phenol, Saturated Solution, Ph 4.5

Phenol, Saturated Solution, Ph 4.5

Synonyms Carbolic acid; Phenic acid

CAS Number: 108-95-2 Molecular Formula: C6H6O Molecular Weight: 94.113 g/mol

Beilstein Registry Number: 6428463 MDL Number: MFCD00002143

Packaging size: 400 mL

SKU 04802517
Price:
$177.65
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Description

Product Description

Phenol, Saturated Solution, Ph 4.5

Application Notes

Phenol Saturated Solution of pH 4.5-5.5 is suitable for denaturing DNA and purifying RNA. It is also used for the purification of RNA from genomic DNA. Although phenol alone will purify RNA in these extractions, it is preferable to include some chloroform. In the complete absence of chloroform, messenger RNA can collect with the DNA at the interphase. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many purposes, a 5:1 ratio of phenol:chloroform is a recommended starting point.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Purification of RNA

Specifications
SKU 04802517
Alternate Names Carbolic acid; Phenic acid
Application Notes Phenol Saturated Solution of pH 4.5-5.5 is suitable for denaturing DNA and purifying RNA. It is also used for the purification of RNA from genomic DNA. Although phenol alone will purify RNA in these extractions, it is preferable to include some chloroform. In the complete absence of chloroform, messenger RNA can collect with the DNA at the interphase. The ratio of phenol to chloroform can vary from no chloroform to a 1:1 mixture, depending on the individual tissue and the character of the RNA being purified. For many purposes, a 5:1 ratio of phenol:chloroform is a recommended starting point.
Auto Ignition 1319° F (USCG, 1999)
Beilstein Registry Number 6428463
Boiling Point 359.1° F at 760 mm Hg (EPA, 1998)
Density 1.0722 at 68° F (EPA, 1998)
Flash Point 174.2° F (EPA, 1998)
Format solution
Hazard Statements H311-H314
Applications Purification of RNA
MDL # MFCD00002143
Melting Point 109° F (EPA, 1998)
Molecular Formula C6H6O
Molecular Weight 94.113 g/mol
Pack Size 400 mL
pH about 6.0 (aq soln)
Physical Appearance Clear; Colorless Solution Capped with a Layer of Buffer
Product Families Description Phenol
Product Overview Phenol, Saturated Solution, Ph 4.5
Purity ≥99%
RTECS Number SJ3325000
Solubility 9 % at 77° F (NIOSH, 2016)
Storage and Handling +4 °C; protect from light
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
UV Visible Absorbance OD330: ≤0.20; OD405: ≤0.10; OD510 ≤0.10
Vapor Density 3.24 (EPA, 1998) (Relative to Air)
Vapor Pressure 0.3513 mm Hg at 77° F (EPA, 1998)