Gly-Phe-7-Amino-4-Trifluoromethylcoumarin

Gly-Phe-7-Amino-4-Trifluoromethylcoumarin

Synonyms Gly-Phe-AFC; GF-AFC

CAS Number: Not yet assigned Molecular Formula: C21H18F3N3O4

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SKU 03AFC033-CF
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Description

Product Description

Gly-Phe-7-Amino-4-Trifluoromethylcoumarin

Application Notes

Gly-Phe-AFC is a fluorogenic, cell-permeant peptide substrate use to measure number of live cells in cytotoxic assay. It enters intact cells where it is cleaved to generate a fluorescent signal proportional to the number of living cells. This live-cell protease activity marker becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. It is also used as substrate for measuring Dipeptidyl peptidase1 (DPP1) activity. DPP1 is a lysosomal cysteinyl protease of the papain family involved in the intracellular degradation of proteins.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Substrate in Cytotoxicity Assay||Substrate in measurement of Dipeptidyl peptidase1 activity

Specifications
SKU 03AFC033-CF
Alternate Names Gly-Phe-AFC; GF-AFC
Application Notes Gly-Phe-AFC is a fluorogenic, cell-permeant peptide substrate use to measure number of live cells in cytotoxic assay. It enters intact cells where it is cleaved to generate a fluorescent signal proportional to the number of living cells. This live-cell protease activity marker becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. It is also used as substrate for measuring Dipeptidyl peptidase1 (DPP1) activity. DPP1 is a lysosomal cysteinyl protease of the papain family involved in the intracellular degradation of proteins.
Format powder
Applications Substrate in Cytotoxicity Assay||Substrate in measurement of Dipeptidyl peptidase1 activity
Molecular Formula C21H18F3N3O4
Physical Appearance White powder
Product Families Description Gly-Phe-7-amino-4-trifluoromethylcoumarin
Product Overview Gly-Phe-7-Amino-4-Trifluoromethylcoumarin
Protein or Enzyme Type Activators, Inhibitors & Substrates
Storage and Handling Keep cool and dry
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.