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Kanamycin Monosulfate

Kanamycin Monosulfate

Synonyms Kanamycin A; Kanamycin sulfate salt

CAS Number: 25389-94-0 Molecular Formula: C18H36N4O11 • H2SO4 Molecular Weight: 582.575 g/mol

EC Number: 246-933-9 MDL Number: MFCD00070253

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SKU 02199853-CF
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Description

Product Description

Kanamycin Monosulfate

Application Notes

Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Antibiotic||Inhibitor of protein synthesis

Specifications
SKU 02199853-CF
Application Notes Kanamycin sulfate is a broad spectrum aminoglycoside-antibiotic derived from Streptomyces kanamyceticus. It is used as an additive in culture media for the isolation of group D streptococci on Kanamycin Esculin Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene.
Biochemical Physiological Actions Kanamycin is an aminoglycoside antibiotic. Aminoglycosides work by binding to the bacterial 30S ribosomal subunit, causing misreading of t-RNA, leaving the bacterium unable to synthesize proteins vital to its growth. Specifically Kanamycin binds to four nucleotides of 16S rRNA and a single amino acid of protein S12. This interferes with decoding site in the vicinity of nucleotide 1400 in 16S rRNA of 30S subunit. This region interacts with the wobble base in the anticodon of tRNA. This leads to interference with the initiation complex, misreading of mRNA so incorrect amino acids are inserted into the polypeptide leading to nonfunctional or toxic peptides and the breakup of polysomes into nonfunctional monosomes.
EC Number 246-933-9
Grade USP Grade
Hazard Statements H360
Applications Antibiotic||Inhibitor of protein synthesis
Loss on Drying <4%
Molecular Formula C18H36N4O11 • H2SO4
Molecular Weight 582.575 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance White Powder
Potency >750 µg/mg
Product Families Description Kanamycin monosulfate
Product Overview Kanamycin Monosulfate
Residue on Ignition <1%
RTECS Number NZ3225030
Safety Symbol GHS08
Source Streptomyces kanamycetikus
Storage and Handling Store at Room Temperature (15-30 °C).
Typical Working Concentration cell culture applications at 100 mg/mL.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.