Alkaline Phosphatase

Alkaline Phosphatase

Synonyms Orthophosphoric Monoester Phosphohydrolase

CAS Number: 9001-78-9 Molecular Weight: 496.569 g/mol

EC Number: 232-631-4 MDL Number: MFCD00131849

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SKU 02199817-CF
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Description

Product Description

Alkaline Phosphatase

Application Notes

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Conjugation||Protein Labelling

Specifications
SKU 02199817-CF
Alternate Names Orthophosphoric Monoester Phosphohydrolase
Application Notes Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. It may be used for protein labeling when high sensitivity is required.
Biochemical Physiological Actions Alkaline phosphatase, from Escherichia coli, is a dimeric, non-glycosylated protein which mainly reside in the periplasmic space. Three known isoforms exist. The enzyme requires zinc, and is activated by magnesium. E. coli akaline phosphatase has a broad specificity for phosphate esters.
EC Number 232-631-4
Format Ammonium Sulfate Suspension
Applications Conjugation||Protein Labelling
MDL # MFCD00131849
Molecular Weight 496.569 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Suspension
Product Families Description Alkaline Phosphatase
Product Overview Alkaline Phosphatase
Source E. Coli
Specific Activity ≥30 u/mg protein , Activity- ~1000 u/mL
Storage and Handling Store at +4 °C; do not freeze.
Unit Definition One unit will hydrolyze 1 µmole of 4-nitrophenyl phosphate per minute at pH 10.4, 37 °C.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.