Ficin

Ficin

Synonyms Ficus proteinase; ficus protease; E.C. 3.4.22.3;Debricin; E.C. 3.4.4.12; Ficain; Higueroxyl Delabarre

CAS Number: 9001-33-6

EC Number: 232-599-1 MDL Number: MFCD00081597

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SKU 02198761-CF
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Description

Product Description

Ficin

Application Notes

Immobilized Ficin was specifically designed for cleavage of mouse IgG1 into F(ab')2 or Fab fragments. Ficin will generate mouse IgG1 F(ab')2 and Fab fragments in the presence of 1 mM cysteine and 10 mM cysteine, respectively. Fragment generation from other species and isotypes may also be possible through modification of the cysteine concentration and other digestion parameters.Ficin isolated from fig latex, is a sulfhydryl protease with a similar active site to bromelain and papain. These enzymes vary in kinetics and stereochemical selectivity and can generate F(ab')2 and Fab fragments. However, yields and immunoreactivity are better when digesting mouse IgG1 with ficin. Ficin acts on bonds involving uncharged and/or aromatic amino acids.The immobilization of ficin enhances stability against denaturation, heat and autolysis. Immobilization also eliminates any potential for antibody-enzyme adducts that cause continued sample digestion. In addition to these advantages, immobilization allows the ficin to be reused.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Cleavage of mouse IgG

Specifications
SKU 02198761-CF
Alternate Names Ficus proteinase; ficus protease; E.C. 3.4.22.3;Debricin; E.C. 3.4.4.12; Ficain; Higueroxyl Delabarre
Application Notes Immobilized Ficin was specifically designed for cleavage of mouse IgG1 into F(ab')2 or Fab fragments. Ficin will generate mouse IgG1 F(ab')2 and Fab fragments in the presence of 1 mM cysteine and 10 mM cysteine, respectively. Fragment generation from other species and isotypes may also be possible through modification of the cysteine concentration and other digestion parameters.Ficin isolated from fig latex, is a sulfhydryl protease with a similar active site to bromelain and papain. These enzymes vary in kinetics and stereochemical selectivity and can generate F(ab')2 and Fab fragments. However, yields and immunoreactivity are better when digesting mouse IgG1 with ficin. Ficin acts on bonds involving uncharged and/or aromatic amino acids.The immobilization of ficin enhances stability against denaturation, heat and autolysis. Immobilization also eliminates any potential for antibody-enzyme adducts that cause continued sample digestion. In addition to these advantages, immobilization allows the ficin to be reused.
Biochemical Physiological Actions Ficin can be used to generate high yielding F (ab′)2 fragments from mouse IgG1. Ficin is classified as a thiol protease. It contains a single reactive cysteine at its active site. The amino acid homology of the active site is similar to that of papain. Ficin will cleave proteins at the carboxyl side of Gly, Ser, Thr, Met, Lys, Arg, Tyr, Ala, Asn, and Val. The reported Km for the chromogenic substrate pGlu-Phe-Leu-p-nitroanilide is 0.43 mM. Ficin is inhibited by iodoacetamide, iodoacetic acid, N-ethylmaleimide, mercuric chloride, DFP (diisopropyl fluorophosphate), TLCK (Na-p-Tosyl-lysine chloromethyl ketone), and TPCK (N-Tosyl-L-phenylalanine chloromethyl ketone).
EC Number 232-599-1
Extinction Coefficient 21.0 (280 nm)(Lit.)
Hazard Statements H315-H319-H334-H335
Isoelectric Point 9.0 (Lit.)
Applications Cleavage of mouse IgG
MDL # MFCD00081597
Personal Protective Equipment Dust mask , Eyeshields, Faceshields, Gloves
Physical Appearance Yellow powder
Product Families Description Ficin
Product Overview Ficin
RTECS Number LK3675000
Safety Symbol GHS07, GHS08
Specific Activity ≥200 Bapa u/g
Storage and Handling Store at +4°C; Desiccate.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.