Dino

3,3,5,5-Tetramethylbenzidine Liquid Substrate

3,3,5,5-Tetramethylbenzidine Liquid Substrate

Synonyms TMB; 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

CAS Number: 54827-17-7 Molecular Weight: 240.35 g/mol

Packaging size: 1 L

SKU 0219602591
Price:
$661.25
In Stock
Request a quote
Description

Product Description

3,3,5,5-Tetramethylbenzidine Liquid Substrate

Application Notes

TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Substrate for horseradish peroxidase

Specifications
SKU 0219602591
Application Notes TMB is a substrate for horseradish peroxidase. Forms a soluble blue end product that may be read at 370 nm or 655 nm. The TMB reaction may be stopped with 0.5 M H2SO4, resulting in a yellow color, and read at 450 nm. Recommended for ELISA (microwell) procedures, not recommended for membrane applications. 3,3′,5,5′-Tetramethylbenzidine has been used in a study to demonstrate that by measuring substrate absorption values off the absorption maximum, the measuring range of any immunoenzymometric assay can be extended significantly without jeopardizing assay characteristics. 3,3′,5,5′-Tetramethylbenzidine has also been used in a study to understand the formation of CuS concave superstructures with peroxidase-like activity.
Format Stabilized; ready-to-use solution
Hazard Statements H226-H302-H312-H332-H370
Applications Substrate for horseradish peroxidase
Melting Point 334 to 336° F (NTP, 1992)
Molecular Weight 240.35 g/mol
Pack Size 1 L
Physical Appearance Liquid
Product Families Description 3,3',5,5'-Tetramethylbenzidine
Product Overview 3,3,5,5-Tetramethylbenzidine Liquid Substrate
Safety Symbol GHS02, GHS07, GHS08
Solubility less than 1 mg/mL at 68° F (NTP, 1992)
Storage and Handling Store at +4 °C. This substrate is very light-sensitive and should be protected from direct sunlight or UV sources.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.