Leupeptin Hydrochloride

Leupeptin Hydrochloride

Synonyms Acetyl-Leu-Leu-Arg-al hydrochloride

CAS Number: 24125-16-4 Molecular Formula: C20H38N6O4·HCl Molecular Weight: 463.02 g/mol

MDL Number: MFCD00133457

Configure Your Product:

SKU 02195624-CF
Price:
$37.35
In Stock
Request a quote
Description

Product Description

Leupeptin Hydrochloride

Application Notes

Leupeptin Hydrochloride is used in immunoblotting and acts as a Protease inhibitor.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Immunoblotting||Protease Inhibitor

Specifications
SKU 02195624-CF
Alternate Names Acetyl-Leu-Leu-Arg-al hydrochloride
Analysis Note Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined by HPLC should take into account the three main peaks. Majority of contaminating peptide is racemized leupeptin. The primary mechanism of inactivation is racemization of the L-arginal; the D-arginal form is totally inactive. If the aldehyde is oxidized but retains its L-configuration, the resulting compound does have some inhibitory activity.
Application Notes Leupeptin Hydrochloride is used in immunoblotting and acts as a Protease inhibitor.
Biochemical Physiological Actions Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)
Applications Immunoblotting||Protease Inhibitor
MDL # MFCD00133457
Molecular Formula C20H38N6O4·HCl
Molecular Weight 463.02 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White to off-white powder
Product Families Description Leupeptin hydrochloride
Product Overview Leupeptin Hydrochloride
Purity ≥60%
Storage and Handling Store at -20 °C; desiccate; protect from light.
Typical Working Concentration Effective concentration is typically 10-100 uM.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.