Leupeptin Hydrochloride is used in immunoblotting and acts as a Protease inhibitor.
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
|Analysis Note||Leupeptin gives multiple peaks on HPLC due to equilibria among three forms in solution. Purity determined by HPLC should take into account the three main peaks. Majority of contaminating peptide is racemized leupeptin. The primary mechanism of inactivation is racemization of the L-arginal; the D-arginal form is totally inactive. If the aldehyde is oxidized but retains its L-configuration, the resulting compound does have some inhibitory activity.|
|Application Notes||Leupeptin Hydrochloride is used in immunoblotting and acts as a Protease inhibitor.|
|Biochemical Physiological Actions||Inhibitor of serine and cysteine proteases. Inhibits plasmin, trypsin, papain, calpain, and cathepsin B. Does not inhibit pepsin, cathepsins A and D, thrombin, or α-chymotrypsin. Effective concentration 10-100 μM. There have been numerous studies using leupeptin to protect against hearing loss caused by acoustic overstimulation or the ototoxic antibiotic gentamicin. (Loss of cochlear hair cells is believed to be mediated by calpain.)|
|Molecular Weight||463.02 g/mol|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|Physical Appearance||White to off-white powder|
|Product Families Description||Leupeptin hydrochloride|
|Product Overview||Leupeptin Hydrochloride|
|Storage and Handling||Store at -20 °C; desiccate; protect from light.|
|Typical Working Concentration||Effective concentration is typically 10-100 uM.|
|Usage Statement||Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.|