Trypan Blue

Trypan Blue

Synonyms Direct Blue 14; Niagara Blue 3B; Chlorazol blue 3B; Benzo blue 3B; Dianil blue H3G; Congo blue 3B; Naphthamine blue 3BX; Benzamine blue 3B; Azidine blue 3B; Diamine Blue 3B; C.I. 23850

CAS Number: 72-57-1 Molecular Formula: C34H24N6O14S4Na4 Molecular Weight: 960.793 g/mol

Beilstein Registry Number: 4360496 EC Number: 200-786-7 MDL Number: MFCD00003969

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SKU 02195532-CF
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Description

Product Description

Trypan Blue

Application Notes

Trypan blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system.Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Staining||Cytotoxicity assays

Specifications
SKU 02195532-CF
Alternate Names Direct Blue 14; Niagara Blue 3B; Chlorazol blue 3B; Benzo blue 3B; Dianil blue H3G; Congo blue 3B; Naphthamine blue 3BX; Benzamine blue 3B; Azidine blue 3B; Diamine Blue 3B; C.I. 23850
Application Notes Trypan blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system.Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
Beilstein Registry Number 4360496
Color Index 23850
EC Number 200-786-7
Format powder
Hazard Statements H350
Applications Staining||Cytotoxicity assays
MDL # MFCD00003969
Melting Point greater than 572° F (NTP, 1992)
Molecular Formula C34H24N6O14S4Na4
Molecular Weight 960.793 g/mol
Personal Protective Equipment Eyeshields, full-face particle respirator , Gloves, respirator cartridge, respirator filter
Physical Appearance Dark Green Powder
Product Families Description Trypan Blue
Product Overview Trypan Blue
RTECS Number QJ6475000
Safety Symbol GHS08
Solubility 1 to 10 mg/mL at 68° F (NTP, 1992)
Storage and Handling Store at Room Temperature (15-30 °C).
Typical Working Concentration For Viable Cell Counting: 0.4% (w/v)
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance λ max (MeOH) 234-7, 315-9, 600-609 nm