Peroxidase

Peroxidase

Synonyms HRP; Donor:hydrogen-peroxide oxidoreductase; E.C. 1.11.1.7; Horseradish peroxidase; Peroxidase from horseradish

CAS Number: 9003-99-0

EC Number: 232-668-6 MDL Number: MFCD00071339

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SKU 02195373-CF
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Description

Product Description

Peroxidase

Application Notes

Horseradish peroxidase is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens. HRP is the most desired label for antibodies since it is the smallest and most stable of the three most popular enzyme labels (HRP, alkaline phosphatase, and B–galactosidase) and its glycosylation leads to lower non–specific binding. It is also useful for tracing neural connections. These include motor and sensory innervation of peripheral organs, and connections of peripheral nerves, ganglia and individual dorsal roots. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross–linkers.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

ELISA||Immunoblotting||Immunohistochemistry

Specifications
SKU 02195373-CF
Alternate Names HRP; Donor:hydrogen-peroxide oxidoreductase; E.C. 1.11.1.7; Horseradish peroxidase; Peroxidase from horseradish
Analysis Note RZ Definition: The ratio of the absorbance at 403 nm to the absorbance at 275 nm. This value is an expression of the ratio of hemin to protein content.
Application Notes Horseradish peroxidase is used in biochemistry applications such as western blots, ELISA and Immunohistochemistry. It is used to amplify a weak signal and increase detectability of a target molecule, such as a protein. HRP labeled immunoglobulins are used as probes for the demonstration of tissue antigens and in enzyme immunoassay (EIA) systems for determination of soluble and insoluble antigens. HRP is the most desired label for antibodies since it is the smallest and most stable of the three most popular enzyme labels (HRP, alkaline phosphatase, and B–galactosidase) and its glycosylation leads to lower non–specific binding. It is also useful for tracing neural connections. These include motor and sensory innervation of peripheral organs, and connections of peripheral nerves, ganglia and individual dorsal roots. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed cross–linkers.
Biochemical Physiological Actions When incubated with a substrate, horseradish peroxidase produces a coloured, fluorimetric, or luminescent derivative of the labeled molecule, allowing quantification. HRP reacts with hydrogen peroxide to form a [HRP-H2O2] complex which can oxidize a wide variety of hydrogen donors.
EC Number 232-668-6
Extinction Coefficient 100 (403 nm)(Lit.)
Format Crystalline powder
Isoelectric Point 7.2(Lit.); Isozymes range from 3.0–9.0(Lit)
Applications ELISA||Immunoblotting||Immunohistochemistry
MDL # MFCD00071339
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Reddish Brown Crystalline Powder
Product Families Description Peroxidase
Product Overview Peroxidase
Source Horseradish(Amoracia rusticana)
Specific Activity ≥250 u/mg solid
Specificity Activity is observed with H2O2; MeOOH and EtOOH.
Storage and Handling Store at -0 °C.
Typical Working Concentration For assay of rate of decomposition hydrogen peroxidide by peroxidase with pyrogallol: 0.50% (w/w) Hydrogen peroxide solution (H2O2); Prepare 50 mL in deionized water using a 30% Hydrogen peroxide solution. RZ Determination=0.5 mg/mL solution of horseradish peroxidase.
Unit Definition That amount of enzyme which catalyses the production of one milligram of purpurogallin in 20 seconds at 20 °C. at a pH of 6.0.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.