Dino

Lysozyme Type VI

Lysozyme Type VI

Synonyms Muramidase; Mucopeptide N-acetylmuramoylhydrolase

CAS Number: 9001-63-2 Molecular Weight: 2235.597 g/mol

EC Number: 232-620-4 MDL Number: MFCD00131557

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SKU 02195303-CF
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Description

Product Description

Lysozyme Type Vi

Application Notes

Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Cell Lysis

Specifications
SKU 02195303-CF
Application Notes Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification.
Biochemical Physiological Actions Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
EC Number 232-620-4
Extinction Coefficient 26.4 (280 nm) (Lit.)
Format Type VI; 3X Crystallized; Salt-Free; Albumin-Free; Lyophilized
Grade Molecular Biology Reagent
Isoelectric Point pH 11.0 (lit)
Applications Cell Lysis
Molecular Weight 2235.597 g/mol
Pack Size No
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White to Off-white Powder
Product Families Description Lysozyme
Product Overview Lysozyme Type Vi
RTECS Number OL5987000
Source Chicken eggwhite
Specific Activity ≥23,500 Shugar units/mg
Storage and Handling -20 °C
Unit Definition One unit will produce a decrease in A450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.