Lysozyme Type VI
Description
Product Description
Lysozyme Type Vi
Application Notes
Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification.
Usage Statement
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Key Applications
Cell Lysis
Specifications
| SKU | 02195303-CF |
| Alternate Names | Muramidase; Mucopeptide N-acetylmuramoylhydrolase |
| Application Notes | Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification. |
| Biochemical Physiological Actions | Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M). |
| EC Number | 232-620-4 |
| Extinction Coefficient | 26.4 (280 nm) (Lit.) |
| Format | Type VI; 3X Crystallized; Salt-Free; Albumin-Free; Lyophilized |
| Grade | Molecular Biology Reagent |
| Isoelectric Point | pH 11.0 (lit) |
| Applications | Cell Lysis |
| MDL # | MFCD00131557 |
| Molecular Weight | 2235.597 g/mol |
| Pack Size | No |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Physical Appearance | White to Off-white Powder |
| Product Families Description | Lysozyme |
| Product Overview | Lysozyme Type Vi |
| RTECS Number | OL5987000 |
| Source | Chicken eggwhite |
| Specific Activity | ≥23,500 Shugar units/mg |
| Storage and Handling | -20 °C |
| Unit Definition | One unit will produce a decrease in A450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate. |
| Usage Statement | Research Use Only (RUO). Ready for CE IVD labeling of clinical applications. |