Fluorescamine

Fluorescamine

Synonyms 4-Phenyl spiro-[furan-2(3H)-1'-(3'-H)-Isobenzofuran]-3,3'-dione; 4-Phenyl spiro-[furan-2(3H), 1-phthalan]-3,3'-dione

CAS Number: 38183-12-9 Molecular Formula: C17H10O4 Molecular Weight: 278.263 g/mol

Beilstein Registry Number: 921143 EC Number: 253-814-5 MDL Number: MFCD00005928

Configure Your Product:

SKU 02195183-CF
Price:
$37.35
In Stock
Request a quote
Description

Product Description

Fluorescamine

Application Notes

Fluorescamine is a reagent for fluorescent assay of amino acids, peptides, proteins and other primary amines. It is also used for fluorometric assays of some proteolytic enzymes. it effectively blocks newly generated amino termini in protein sequence analyses by the Beckman automated sequencer. This product has been used in labeling casein so that it can be used as a substrate for measuring protease activity. Fluorescamine is used in many sensitive detection methods, eg. characterization of poly-L-lysine (pLL)/DNA complexes post-modified with a multivalent hydrophilic polymer, spectrofluorimetric analysis of procaine, detection and quantitation of residual aminopenicillins by HPLC after fluorescamine derivation, determination of lisinopril in human plasma and urine and sulfonamides in honey by HPLC with fluorescence detection.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Fluorometric assay

Specifications
SKU 02195183-CF
Alternate Names 4-Phenyl spiro-[furan-2(3H)-1'-(3'-H)-Isobenzofuran]-3,3'-dione; 4-Phenyl spiro-[furan-2(3H), 1-phthalan]-3,3'-dione
Application Notes Fluorescamine is a reagent for fluorescent assay of amino acids, peptides, proteins and other primary amines. It is also used for fluorometric assays of some proteolytic enzymes. it effectively blocks newly generated amino termini in protein sequence analyses by the Beckman automated sequencer. This product has been used in labeling casein so that it can be used as a substrate for measuring protease activity. Fluorescamine is used in many sensitive detection methods, eg. characterization of poly-L-lysine (pLL)/DNA complexes post-modified with a multivalent hydrophilic polymer, spectrofluorimetric analysis of procaine, detection and quantitation of residual aminopenicillins by HPLC after fluorescamine derivation, determination of lisinopril in human plasma and urine and sulfonamides in honey by HPLC with fluorescence detection.
Beilstein Registry Number 921143
EC Number 253-814-5
Format powder
Applications Fluorometric assay
MDL # MFCD00005928
Molecular Formula C17H10O4
Molecular Weight 278.263 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance White Powder
Product Families Description Fluorescamine
Product Overview Fluorescamine
Storage and Handling Store at Room Temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.