Urea

Urea

Synonyms Carbamide; Isourea; Carbonyldiamine; Ureophil; Urevert; Carbonyl diamide

CAS Number: 57-13-6 Molecular Formula: CH4N2O Molecular Weight: 60.056 g/mol

Beilstein Registry Number: 635724 EC Number: 200-315-5 MDL Number: MFCD00008022

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SKU 02194857-CF
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Description

Product Description

Urea

Application Notes

Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins

Specifications
SKU 02194857-CF
Alternate Names Carbamide; Isourea; Carbonyldiamine; Ureophil; Urevert; Carbonyl diamide
Application Notes Urea is used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies and in the extraction of the mitochondrial complex. It is commonly used to solubilize and denature proteins for denaturing isoelectric focusing and two-dimensional electrophoresis and in acetic acid-urea PAGE gels. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form. Urea is used in cell or tissue culture media to increase the osmolality. Urea has also been used as fertilizer because of the easy availability of nitrogen; in animal feeds; it is reacted with aldehydes to make resins and plastics; condensed with malonic ester to form barbituric acid; used in the paper industry to soften cellulose.
Auto Ignition Not flammable (USCG, 1999)
Beilstein Registry Number 635724
Biochemical Physiological Actions Urea has been shown to act as an aldosterone antagonist in the development of peanut agglutinin binding in cultured embryonic renal collecting duct epithelial cells. Urea is the principal end product of nitrogen metabolism in most mammals, formed by the enzymatic reactions of the Kreb's cycle.
Boiling Point Decomposes (NTP, 1992)
Conductivity 68.4 uMhos (Lit.)
Density 1.34 at 68° F (USCG, 1999)
EC Number 200-315-5
Foreign Activity RNAse; DNAse; Protease: Negative
Format powder
Grade Molecular biology reagent
Heavy Metals Pb: ≤2 ppm
Applications Denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins
MDL # MFCD00008022
Melting Point 275° F (NTP, 1992)
Molecular Formula CH4N2O
Molecular Weight 60.056 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
pH 7.2 (10% solution)
Physical Appearance White Powder
Product Families Description Urea
Product Overview Urea
Purity ≥98%
RTECS Number YR6250000
Solubility >9 [ug/mL]
Storage and Handling Store at Room Temperature (15-30 °C).
Typical Working Concentration The use of 2 g/L urea in the culture of Kluyveromyces marxianus to produce a thermostable extracellular lipase has been described. Urea is typically used at a concentration of 8 M for protein denaturation or solubilization. A final concentration of 5 M urea is commonly used in molecular biology for sequencing gels. To prevent carbamylation, do not heat urea containing buffers above 37 °C.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
UV Visible Absorbance OD260 nm (7 M aq soln): ≤0.2; OD280 (7M aq soln): ≤0.2
Vapor Pressure 1.2X10-5 mm Hg @ 25 deg C