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2-Mercaptoethanol

2-Mercaptoethanol

Synonyms 2-Thioethanol; 2-Hydroxyethylmercaptan; 2-Hydroxy-1-Ethanethiol; Monothioethyleneglycol; β-Mercaptoethanol; BME; Thioethylene glycol; 2-Hydroxyethyl Mercaptan; 1-ethane-2-thiol; 2-ME; 2-thioethanol

CAS Number: 60-24-2 Molecular Formula: C2H6OS Molecular Weight: 78.129 g/mol

Beilstein Registry Number: 773648 EC Number: 200-464-6 MDL Number: MFCD00004890

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SKU 02194834-CF
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Description

Product Description

2-Mercaptoethanol

Application Notes

2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Purification||Reducing agent

Specifications
SKU 02194834-CF
Application Notes 2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Auto Ignition 295°C
Beilstein Registry Number 773648
Boiling Point 315 to 316° F at 742 mm Hg (decomposes) (NTP, 1992)
Density 1.1143 at 68° F (NTP, 1992)
EC Number 200-464-6
Flash Point 165° F (NFPA, 2010)
Format liquid
Grade Molecular biology reagent
Hazard Statements H301-H310 + H330-H315-H317-H318-H410
Applications Purification||Reducing agent
Melting Point -148° F (NTP, 1992)
Molecular Formula C2H6OS
Molecular Weight 78.129 g/mol
Personal Protective Equipment Faceshields, Full-face respirator, Gloves, Goggles, Multi-purpose combination respirator cartridge
Physical Appearance Liquid
Product Families Description 2-Mercaptoethanol
Product Overview 2-Mercaptoethanol
Purity ≥98%
Refractive Index n20/D 1.500(lit.)
RTECS Number KL5600000
Safety Symbol GHS05, GHS06, GHS09
Solubility greater than or equal to 100 mg/mL at 68° F (NTP, 1992)
Storage and Handling Store at Room Temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vapor Density 2.69 (NTP, 1992) (Relative to Air)
Vapor Pressure 1 mm Hg at 68° F (NTP, 1992)