Hepes Sodium Salt

Hepes Sodium Salt

Synonyms 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt; sodium N-(2-hydroxyethyl) piperazine-N'- (2-ethanesulfonate); sodium 4-(2-hydroxyethyl)-1- piperazineethanesulfonate

CAS Number: 75277-39-3 Molecular Formula: C8H17N2O4S·Na Molecular Weight: 260.3

EC Number: 278-169-7 MDL Number: MFCD00036463

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SKU 02194828-CF
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Description

Product Description

HEPES is a zwitterionic organic chemical buffering agent.

Application Notes

HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. It is used in a protein deposition technique in electron microscopy because it did not affect metal substrates.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Buffering agent

Specifications
SKU 02194828-CF
Alternate Names 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt; sodium N-(2-hydroxyethyl) piperazine-N'- (2-ethanesulfonate); sodium 4-(2-hydroxyethyl)-1- piperazineethanesulfonate
Application Notes HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. It is used in a protein deposition technique in electron microscopy because it did not affect metal substrates.
Chemical Class Buffers
Detection Method FTIR
EC Number 278-169-7
Format powder
Grade Molecular Biology Reagent
Applications Buffering agent
Loss on Drying <3.0%
MDL # MFCD00036463
Molecular Formula C8H17N2O4S·Na
Molecular Weight 260.3
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
pH 9.5 - 10.8 (1% aq soln); Useful pH range=6.8-8.2(Lit)
Physical Appearance White Powder
pKa 7.55 at 25 °C (Lit.)
Purity ≥99.0%
Solubility Soluble in water.
Sterilization of Solutions Solutions may be autoclaved under standard conditions
Storage and Handling Store at Room Temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.