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HEPES

HEPES

Synonyms N-2-Hydroxyethylpiperazine-N´-2-ethanesulfonic acid; N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid); 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid

CAS Number: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight: 238.3

Beilstein Registry Number: 883043 EC Number: 230-907-9 MDL Number: MFCD00006158

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SKU 02194827-CF
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$46.45
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Description

Product Description

HEPES is a zwitterionic organic chemical buffering agent.

Application Notes

HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Buffering agent

Specifications
SKU 02194827-CF
Application Notes HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media.
Beilstein Registry Number 883043
Chemical Class Buffers
EC Number 230-907-9
Format powder
Grade Molecular Biology Reagent
Applications Buffering agent
Melting Point 234 °C(Lit.)
Moisture Content <1.0%
Molecular Formula C8H18N2O4S
Molecular Weight 238.3
Personal Protective Equipment Eyeshields, Gloves, respirator filter
pH 4.0 - 5.5 (1% aq soln); Useful pH range=6.8-8.2(Lit)
Physical Appearance White Powder
pKa 7.5 (25 °C) (Lit.)
Purity ≥99%
RTECS Number TL6809000
Solubility Soluble in 10% aq solution(Clear, Colorless Solution).
Sterilization of Solutions Solutions may be autoclaved under standard conditions
Storage and Handling Store at Room Temperature (15-30 °C); desiccate.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance OD250 (10% aq soln) <0.2, OD260 (10% aq soln) <0.1, OD280 (10% aq soln) <0.1