Glycine, molecular biology reagent
Product Description
Glycine
Application Notes
Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology.
Usage Statement
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
Key Applications
Buffer component
| SKU | 02194825-CF |
| Alternate Names | Aminoacetic acid; Glycolixir; Hampshire Glycine; Gly; Aminoethanoic acid; Glycocoll; G |
| Application Notes | Glycine is commonly used in buffer solutions, in electrophoresis and preparative chromatography. A study of the folding of monoclonal antibodies in the presence of glycine and their subsequent purification has been published. The use of glycine in the purification of lipopolysaccharides, lipooligosaccharides, and lipid A has been reported. It is an amino acid for use in cell culture media development applications and existing media formulations. Glycine is commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots, a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. The growth requirements of various microorganisms is reported in the Handbook of Microbiology. |
| Base Catalog Number | 194825 |
| Beilstein Registry Number | 635782 |
| Biochemical Physiological Actions | Glycine is a non-chiral amino acid that can be synthesized in the body from the amino acid serine by Serine Hydroxymethyltransferase. Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors. |
| CAS # | 56-40-6 |
| Density | 1.1607 (NTP, 1992) |
| EC Number | 200-272-2 |
| Format | powder |
| Grade | Molecular biology reagent |
| Applications | Buffer component |
| Loss on Drying | ≤0.5% |
| Melting Point | 451° F (NTP, 1992) |
| Molecular Formula | C2H5NO2 |
| Molecular Weight | 75.067 g/mol |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| pH | 0.2 molar soln in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>: 4.0 |
| Physical Appearance | Powder |
| pKa | 2.35 at 25 °C (Lit.) |
| Product Families Description | Glycine |
| Product Overview | Glycine |
| Purity | ≥99% |
| RTECS Number | MB7600000 |
| Solubility | greater than or equal to 100 mg/mL at 64° F (NTP, 1992) |
| Storage and Handling | Store at Room Temperature (15-30 °C). |
| Usage Statement | Research Use Only (RUO). Ready for CE IVD labeling of clinical applications. |