β-Nicotinamide Adenine Dinucleotide, Oxidized Form

β-Nicotinamide Adenine Dinucleotide, Oxidized Form

Synonyms β-DPN; NAD; Nadide; β-NAD; Diphosphopyridine nucleotide; DPN; Coenzyme 1; Cozymase; Nadide; Adenosine 5'-(trihydrogen diphosphate) P'->5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium inner salt; 3-carbamoyl-1-β-D-ribofuranosylpyridinium hydroxide 5'->5'- ester with adenosine 5'-(trihydrogen pyrophosphate) inner salt

CAS Number: 53-84-9 Molecular Formula: C21H27N7O14P2·×H2O Molecular Weight: 663.43 g/mol

EC Number: 200-184-4 MDL Number: MFCD00036253

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SKU 02194714-CF
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Description

Product Description

β-Nicotinamide Adenine Dinucleotide, Oxidized Form

Application Notes

Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Fluorescent measurement of the oxidized or reduced pyridine nucleotides

Specifications
SKU 02194714-CF
Alternate Names β-DPN; NAD; Nadide; β-NAD; Diphosphopyridine nucleotide; DPN; Coenzyme 1; Cozymase; Nadide; Adenosine 5'-(trihydrogen diphosphate) P'->5'-ester with 3-(aminocarbonyl)-1-β-D-ribofuranosylpyridinium inner salt; 3-carbamoyl-1-β-D-ribofuranosylpyridinium hydroxide 5'->5'- ester with adenosine 5'-(trihydrogen pyrophosphate) inner salt
Application Notes Many metabolites and enzymes of biological interest are present in tissues at low concentrations. With the use of β-NAD as a catalyst intermediate and several enzymes in a multistep system, known as enzyme cycling, much greater sensitivity for detection of these components is achieved. The reduced form, β-NADH, is fluorescent whereas β-NAD is not. This difference in fluorescence provides a sensitive fluorescent measurement of the oxidized or reduced pyridine nucleotides at concentrations down to 10-7 M.
Biochemical Physiological Actions Electron acceptor. β-NAD is a carrier for hydride ion, forming b-NADH. Hydride ion is enzymatically removed from a substrate molecule by the action of dehydrogenases such as malic dehydrogenase and lactic dehydrogenase. Such enzymes catalyze the reversible transfer of a hydride ion from malate or lactate to b-NAD to form the reduced product, b-NADH. Unlike b-NAD which has no absorbance at 340 nm, b-NADH absorbs at 340 nm (EmM = 6.22). The increase in absorbance at 340 nm with the formation of b-NADH is the basis for measurement of activity of many enzymes.
EC Number 200-184-4
Extinction Coefficient EmM = 18.0 (260 nm, pH 7)(Lit.)
Format powder
Grade Cell culture reagent
Applications Fluorescent measurement of the oxidized or reduced pyridine nucleotides
MDL # MFCD00036253
Molecular Formula C21H27N7O14P2·×H2O
Molecular Weight 663.43 g/mol
Physical Appearance White Powder
Product Families Description β-Nicotinamide adenine dinucleotide
Product Overview β-Nicotinamide Adenine Dinucleotide, Oxidized Form
Purity ≥98 %
RTECS Number UU3450000
Storage and Handling -20°C; desiccate
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance λ max (water) 259 ± 3 nm