3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide

3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide

Synonyms MTT; Thiazolyl Blue;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;Methylthiazolyldiphenyl-tetrazolium bromide

CAS Number: 298-93-1 Molecular Formula: C18H16BrN5S Molecular Weight: 414.325 g/mol

Beilstein Registry Number: 4081397 EC Number: 206-069-5 MDL Number: MFCD00011964

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Description

Product Description

3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide

Application Notes

3-(4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Colorimetric assay; Cell viability; cell culture

Specifications
SKU 02194592-CF
Alternate Names MTT; Thiazolyl Blue;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;Methylthiazolyldiphenyl-tetrazolium bromide
Application Notes 3-(4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Beilstein Registry Number 4081397
EC Number 206-069-5
Grade Cell Culture Reagent
Hazard Statements H315-H319-H335-H341
Applications Colorimetric assay; Cell viability; cell culture
MDL # MFCD00011964
Melting Point 195 deg C (decomposes)
Molecular Formula C18H16BrN5S
Molecular Weight 414.325 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Yellow Powder
Product Families Description 3(4,5-Dimethylthiazolyl-2)2,5-diphenyl tetrazolium bromide
Product Overview 3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide
Purity ~98%
RTECS Number XF8060000
Safety Symbol GHS07, GHS08
Solubility Solubility in <a class="pubchem-internal-link CID-8019" href="https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol">2-methoxyethanol</a>, 20 mg/mL; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 20 mg/mL
Storage and Handling +4°C; protect from light
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance 376 ± 2 nm; 242 ± 2 nm λmax (methanol)
Vapor Pressure 5.11X10-11 mm Hg at 25 deg C /Estimated/