Tris Hydrochloride

Tris Hydrochloride

Synonyms Tris(hydroxymethyl)aminomethane hydrochloride; TRIS HCl

CAS Number: 77-86-1 Molecular Formula: C4H11NO3 • HCl Molecular Weight: 121.136 g/mol

Beilstein Registry Number: 3675235 EC Number: 214-684-5 MDL Number: MFCD00012590

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SKU 02194558-CF
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Description

Product Description

Tris Hydrochloride

Application Notes

Tris-HCl is excellent biochemical and biological buffer for all applications where ultra high purity is required. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Specifications
SKU 02194558-CF
Alternate Names Tris(hydroxymethyl)aminomethane hydrochloride; TRIS HCl
Application Notes Tris-HCl is excellent biochemical and biological buffer for all applications where ultra high purity is required. The pH values of all buffers are temperature and concentration dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution. For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
Beilstein Registry Number 3675235
Boiling Point 219-220 deg C at 10 mm Hg
EC Number 214-684-5
Format Crystalline powder
Grade Cell Culture Reagent
MDL # MFCD00012590
Melting Point 171-172 deg C
Molecular Formula C4H11NO3 • HCl
Molecular Weight 121.136 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Gloves
pH pH of 0.1 molar aq soln = 10.4
Physical Appearance White Crystalline Powder
pKa 8.1 (25 °C)(Lit.)
Product Families Description TRIS hydrochloride
Product Overview Tris Hydrochloride
Purity ≥99%
Solubility 79.1 mg/mL in <a class="pubchem-internal-link CID-174" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethylene%20glycol">ethylene glycol</a>; 26 mg/mL in <a class="pubchem-internal-link CID-887" href="https://pubchem.ncbi.nlm.nih.gov/compound/methanol">methanol</a>; 14.6 mg/mL in anhyd <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 22 mg/mL in 95% <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>; 14 mg/mL in <a class="pubchem-internal-link CID-6228" href="https://pubchem.ncbi.nlm.nih.gov/compound/dimethyl-formamide">dimethyl formamide</a>; 20 mg/mL in <a class="pubchem-internal-link CID-180" href="https://pubchem.ncbi.nlm.nih.gov/compound/acetone">acetone</a>; 0.5 mg/mL in <a class="pubchem-internal-link CID-8857" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethyl%20acetate">ethyl acetate</a>; 0.4 mg/mL in olive oil; 0.1 mg/mL in <a class="pubchem-internal-link CID-8078" href="https://pubchem.ncbi.nlm.nih.gov/compound/cyclohexane">cyclohexane</a>; 0.05 mg/mL in <a class="pubchem-internal-link CID-6212" href="https://pubchem.ncbi.nlm.nih.gov/compound/chloroform">chloroform</a>; less than 0.05 mg/mL in <a class="pubchem-internal-link CID-5943" href="https://pubchem.ncbi.nlm.nih.gov/compound/carbon%20tetrachloride">carbon tetrachloride</a>
Storage and Handling Store at Room Temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
UV Visible Absorbance OD260 (5%aq soln) <0.1 ; OD 280 (5%aq soln) <0.1
Vapor Pressure 2.2X10-5 mm Hg at 25 deg C (est)