Bis-Tris

Bis-Tris

Synonyms 2,2-Bis-(hydroxymethyl)-2,2´,2´´- nitrilotriethanol; bis(2-hydroxymethyl)imino-tris (hydroxymethyl)methane; 2-bis(2-Hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propandiol

CAS Number: 6976-37-0 Molecular Formula: C8H19NO5 Molecular Weight: 209.242 g/mol

Beilstein Registry Number: 2205275 EC Number: 230-237-7 MDL Number: MFCD00002853

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SKU 02194546-CF
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Description

Product Description

Bis-Tris

Application Notes

Bis-Tris is suitable for fractionation of hemoglobin. A protocol for the agarose gel electrophoresis of glyoxylated RNA that uses Bis-Tris in the electrophoresis buffer has been described. The use of bis-Tris in polyacrylamide gels for the in-gel derivatization of proteins for cysteine-specific cleavages and MS analysis has been reported. A multiphasic buffer system for the SDS-PAGE analysis of proteins and peptides in the molecular weight range of 1-100 kDa that incorporates bis-Tris as a counterion in the stacking phase has been described. Bis-Tris buffer has been utilized in a study of λ cro repressor self-assembly and dimerization.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Used in electrophoresis buffer

Specifications
SKU 02194546-CF
Alternate Names 2,2-Bis-(hydroxymethyl)-2,2´,2´´- nitrilotriethanol; bis(2-hydroxymethyl)imino-tris (hydroxymethyl)methane; 2-bis(2-Hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propandiol
Application Notes Bis-Tris is suitable for fractionation of hemoglobin. A protocol for the agarose gel electrophoresis of glyoxylated RNA that uses Bis-Tris in the electrophoresis buffer has been described. The use of bis-Tris in polyacrylamide gels for the in-gel derivatization of proteins for cysteine-specific cleavages and MS analysis has been reported. A multiphasic buffer system for the SDS-PAGE analysis of proteins and peptides in the molecular weight range of 1-100 kDa that incorporates bis-Tris as a counterion in the stacking phase has been described. Bis-Tris buffer has been utilized in a study of λ cro repressor self-assembly and dimerization.
Beilstein Registry Number 2205275
EC Number 230-237-7
Extinction Coefficient EM (water): 259 nm = 10; 277 nm = 10 (Lit.)
Format powder
Grade Cell culture reagent
Hazard Statements H315-H319-H335
Applications Used in electrophoresis buffer
MDL # MFCD00002853
Molecular Formula C8H19NO5
Molecular Weight 209.242 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Gloves
Physical Appearance White Powder
pKa 6.5 (25 °C)(Lit.)
Product Families Description BIS-TRIS
Product Overview Bis-Tris
Purity ≥99%
Safety Symbol GHS07
Sterilization of Solutions For sterilization of aqueous solutions, MP Biomedicals recommends sterile filtration with a 0.2 µm filter; however, the product is typically stable for autoclaving.
Storage and Handling Store at Room Temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.