Poly-L-Lysine Hydrobromide

Poly-L-Lysine Hydrobromide

Synonyms PLL HBr

CAS Number: 25988-63-0 Molecular Formula: (C6H12N2O·HBr)n Molecular Weight: 402.54 g/mol

MDL Number: MFCD00237230

Packaging size: 5 mg

SKU 0219454405
Price:
$59.40
In Stock
Request a quote
Description

Product Description

Poly-L-Lysine Hydrobromide

Application Notes

Poly-L-lysine typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. Can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. Other uses for poly-lysine have been reported as follow: 1.Conjugation to methotrexate for increased drug transport. 2.Microencapsulation of islets. 3.Use in simple reproducible procedure for chromosomal preparations from a variety of tissues. 4.Immobilation of living cells in biocompatible semipermeable microcapsules. 5.Poly-L-Lysine In the preparation of polycationic beads. 6.Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Cellular attachment factor in culture applications

Specifications
SKU 0219454405
Alternate Names PLL HBr
Application Notes Poly-L-lysine typically used as a coating substrate for culture dishes, slides, etc. It enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When adsorbed to the culture surface, poly-lysine increases the number of positively charged sites available for cell binding. Can be used as a coating substrate since poly-lysine is a nonspecific attachment factor for cells; however, certain cells can digest poly-lysine. Other uses for poly-lysine have been reported as follow: 1.Conjugation to methotrexate for increased drug transport. 2.Microencapsulation of islets. 3.Use in simple reproducible procedure for chromosomal preparations from a variety of tissues. 4.Immobilation of living cells in biocompatible semipermeable microcapsules. 5.Poly-L-Lysine In the preparation of polycationic beads. 6.Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.
Certifications γ-irradiated
Format powder
Grade Cell culture reagent; γ-Irradiated
Applications Cellular attachment factor in culture applications
MDL # MFCD00237230
Molecular Formula (C6H12N2O·HBr)n
Molecular Weight 402.54 g/mol
Pack Size 5 mg
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Cream Powder
Product Families Description Poly-L-lysine hydrobromide
Product Overview Poly-L-Lysine Hydrobromide
Sterility γ-Irradiated
Storage and Handling -20 °C.
Typical Working Concentration Typically, the effective coating solution concentration for culture dishes will be 0.3 to 0.5% and for histology slides at 0.1%. Recommended as a cell culture substratum when using 0.5 mL of a 0.1 mg/mL solution to coat 25 cm2. Optimal conditions for attachment must be determined for each cell line and application.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.