Puromycin Dihydrochloride

Puromycin Dihydrochloride

Synonyms Stylomycin ; 6-dimethylamino-9-(3'-deoxy-3'-(p-methoxy-L-phenylalanyamino)- B-D-ribofuranosyl)-purine; 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride

CAS Number: 58-58-2 Molecular Formula: C22H29N7O5·2HCl Molecular Weight: 544.434 g/mol

Beilstein Registry Number: 3853613 EC Number: 200-387-8 MDL Number: MFCD00012691

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SKU 02194539-CF
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Description

Product Description

Puromycin Dihydrochloride

Application Notes

Puromycin can be used in vitro as a selection agent for cells transfected with puromycin N-acetyl transferase gene (pac). It causes premature chain termination in protein synthesis, and is an inhibitor of aminopeptidase and enkephalinase.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Inhibitor of aminopeptidase and enkephalinase.

Specifications
SKU 02194539-CF
Alternate Names Stylomycin ; 6-dimethylamino-9-(3'-deoxy-3'-(p-methoxy-L-phenylalanyamino)- B-D-ribofuranosyl)-purine; 3′-[α-Amino-p-methoxyhydrocinnamamido]-3′-deoxy-N,N-dimethyladenosine dihydrochloride
Application Notes Puromycin can be used in vitro as a selection agent for cells transfected with puromycin N-acetyl transferase gene (pac). It causes premature chain termination in protein synthesis, and is an inhibitor of aminopeptidase and enkephalinase.
Beilstein Registry Number 3853613
Biochemical Physiological Actions Mode of Action: Puromycin inhibits protein synthesis by causing premature chain termination acting as an analog of the 3'-terminal end of the aminoacyl-tRNA.Mode of Resistance: Puromycin acetyltransferase is an effective resistance gene.Antimicrobial Spectrum: This product is active against gram-positive microorganisms, less active against acid-fast bacilli and more weakly active against gram-negative microorganisms. Puromycin can prevent growth of bacteria, protozoa, algae and mammalian cells and acts quickly, killing 99% of cells within 2 days.
EC Number 200-387-8
Format powder
Grade Cell Culture Reagent
Hazard Statements H302
Applications Inhibitor of aminopeptidase and enkephalinase.
MDL # MFCD00012691
Molecular Formula C22H29N7O5·2HCl
Molecular Weight 544.434 g/mol
Personal Protective Equipment Dust mask , Eyeshields, Gloves
Physical Appearance Off-white Powder
Product Families Description Puromycin dihydrochloride
Product Overview Puromycin Dihydrochloride
RTECS Number AU7355000
Safety Symbol GHS07
Solubility Soluble (NTP, 1992)
Storage and Handling -20 °C.
Typical Working Concentration recommended working concentration in eukaryotic cell culture is 1-10 µg/mL
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance λ max (0.1N HCl) 268 ± 2 nm