Hygromycin B

Hygromycin B

Synonyms O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene- (1->2-3)-O-b-D- talopyranosyl- (1->5)- 2-deoxy- N3-methyl-D-streptamine

CAS Number: 31282-04-9 Molecular Formula: C20H37N3O13

EC Number: 250-545-5 MDL Number: MFCD06795479

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SKU 02194170-CF
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Description

Product Description

Hygromycin B

Application Notes

Hygromycin B is used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

In gene transfer experiments as a selection agent.

Specifications
SKU 02194170-CF
Alternate Names O-6-Amino-6-deoxy-L-glycero-D-galacto-heptopyranosylidene- (1->2-3)-O-b-D- talopyranosyl- (1->5)- 2-deoxy- N3-methyl-D-streptamine
Application Notes Hygromycin B is used to select drug-resistant stable transfectants after transfer of the hygromycin phosphotransferase gene in a variety of cell culture applications. It is an aminoglycoside antibiotic produced by the bacterium Streptomyces hygroscopicus that kills bacteria, fungi and higher eukaryotic cells by inhibiting protein synthesis. It is used for the selection and maintenance of prokaryotic and eukaryotic cells that contain the hygromycin resistance gene.
Biochemical Physiological Actions Blocks polypeptide synthesis and inhibits elongation. Specifically, it inhibits protein synthesis by interfering with translocation of the 70S ribosome and inducing misreading of the mRNA template.
EC Number 250-545-5
Hazard Statements H300 +H310 + H330-H318-H334
Applications In gene transfer experiments as a selection agent.
MDL # MFCD06795479
Molecular Formula C20H37N3O13
Physical Appearance White Powder
pKa 7.1
Product Families Description Hygromycin B
Product Overview Hygromycin B
Purity ~95%
RTECS Number WK2130000
Safety Symbol GHS05, GHS06, GHS08
Source Streptomyces hygroscopicus
Specific Activity >1000 u/mg
Storage and Handling Store at +4 °C.
Typical Working Concentration When using this product for mammalian cell selection the working concentration is generally between 50 mg/mL and 1 mg/mL, but at times may go as high as 1 mg/mL. The appropriate concentration for use must be determined empirically for each cell type.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.