Dino

Isopropyl-Β-D-Thiogalactopyranoside

Isopropyl-Β-D-Thiogalactopyranoside

Synonyms IPTG; Isopropyl β-D-thiogalactoside; l-Propyl-b-D-thiogalactopyranoside

CAS Number: 367-93-1 Molecular Formula: C9H18O5S Molecular Weight: 238.298 g/mol

Beilstein Registry Number: 4631 EC Number: 206-703-0 MDL Number: MFCD00063273

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SKU 02194029-CF
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Description

Product Description

Isopropyl-Β-D-Thiogalactopyranoside

Application Notes

Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

β-Galactoside inducer; protein expression

Specifications
SKU 02194029-CF
Application Notes Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Beilstein Registry Number 4631
EC Number 206-703-0
Grade Molecular Biology
Hazard Statements H319-H351
Applications β-Galactoside inducer; protein expression
Melting Point 121 °C
Molecular Formula C9H18O5S
Molecular Weight 238.298 g/mol
Optical Rotation (c=1, water)-26 ° to -33 °
Physical Appearance White Powder
Product Families Description Isopropyl-β-D-thiogalactopyranoside
Product Overview Isopropyl-Β-D-Thiogalactopyranoside
Purity ≥99%
Safety Symbol GHS07,GHS08
Sterilization of Solutions Solutions can be sterile filtered using a 0.2 mm filter.
Storage and Handling Store at -0 °C; desiccate.
Typical Working Concentration The final concentration typically used in indicator plates is 0.2 mM.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.