Ethidium Bromide
Product Description
Ethidium Bromide
Application Notes
Ethidium bromide is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Usage Statement
Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Key Applications
Fluorometric detection of double stranded nucleic acids in gel electrophoresis
| SKU | 02193993-CF |
| Application Notes | Ethidium bromide is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. |
| Beilstein Registry Number | 3642536 |
| Biochemical Physiological Actions | Ethidium Bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells. |
| Density | 0.34 g/cm³ |
| EC Number | 214-984-6 |
| Extinction Coefficient | EmM= 0.20-0.5; 285 nm, EmM = 5.0-10.0; 316 nm, EmM = 40.0 (water), EmM= 5.76 (0.66 M glycine buffer)(Lit.) |
| Flash Point | >100 °C |
| Format | powder |
| Grade | Molecular biology reagent |
| Hazard Statements | H302-H330-H341 |
| Applications | Fluorometric detection of double stranded nucleic acids in gel electrophoresis |
| Melting Point | 260-262 deg C (decomposes) |
| Molecular Formula | C21H20N3Br |
| Molecular Weight | 394.316 g/mol |
| Personal Protective Equipment | Eyeshields, Faceshields, Full-face particle respirator, Gloves, Respirator cartridge, Respirator filter |
| Physical Appearance | Dark Reddish/Purple Powder |
| Product Families Description | Ethidium bromide |
| Product Overview | Ethidium Bromide |
| Purity | ≥98% |
| RTECS Number | SF7950000 |
| Safety Symbol | GHS06, GHS08 |
| Solubility | Solubility in <a class="pubchem-internal-link CID-8019" href="https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol">2-methoxyethanol</a> is 20 mg/mL; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 2 mg/mL |
| Storage and Handling | Store at Room Temperature (15-30 °C). |
| Usage Statement | Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Vapor Pressure | 1.2X10-12 mm Hg at 25 deg C /Estimated/ |