Ethidium Bromide

Ethidium Bromide

Synonyms 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide; Homidium bromide; EtBr; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide

CAS Number: 1239-45-8 Molecular Formula: C21H20N3Br Molecular Weight: 394.316 g/mol

Beilstein Registry Number: 3642536 EC Number: 214-984-6 MDL Number: MFCD00011724

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SKU 02193993-CF
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Description

Product Description

Ethidium Bromide

Application Notes

Ethidium bromide is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Fluorometric detection of double stranded nucleic acids in gel electrophoresis

Specifications
SKU 02193993-CF
Alternate Names 2,7-Diamino-10-ethyl-9-phenylphenanthridinium bromide; Homidium bromide; EtBr; 3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide
Application Notes Ethidium bromide is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/mL). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.
Beilstein Registry Number 3642536
Biochemical Physiological Actions Ethidium Bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It also acts as an RNA polymerase inhibitor, and in separation of high molecular weight DNA's. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.
Density 0.34 g/cm³
EC Number 214-984-6
Extinction Coefficient EmM= 0.20-0.5; 285 nm, EmM = 5.0-10.0; 316 nm, EmM = 40.0 (water), EmM= 5.76 (0.66 M glycine buffer)(Lit.)
Flash Point >100 °C
Format powder
Grade Molecular biology reagent
Hazard Statements H302-H330-H341
Applications Fluorometric detection of double stranded nucleic acids in gel electrophoresis
MDL # MFCD00011724
Melting Point 260-262 deg C (decomposes)
Molecular Formula C21H20N3Br
Molecular Weight 394.316 g/mol
Personal Protective Equipment Eyeshields, Faceshields, Full-face particle respirator, Gloves, Respirator cartridge, Respirator filter
Physical Appearance Dark Reddish/Purple Powder
Product Families Description Ethidium bromide
Product Overview Ethidium Bromide
Purity ≥98%
RTECS Number SF7950000
Safety Symbol GHS06, GHS08
Solubility Solubility in <a class="pubchem-internal-link CID-8019" href="https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol">2-methoxyethanol</a> is 20 mg/mL; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 2 mg/mL
Storage and Handling Store at Room Temperature (15-30 °C).
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Vapor Pressure 1.2X10-12 mm Hg at 25 deg C /Estimated/