Dino

Ribonuclease A

Ribonuclease A

Synonyms Ribonuclease pancreatic; Pancreatic Rnase; Alkaline ribonuclease; RNase A; Ribonuclease I; Ribonucleate 3′-pyrimidinooligonucleotidohydrolase

CAS Number: 9001-99-4

EC Number: 232-646-6 MDL Number: MFCD00132181

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SKU 02193980-CF
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Description

Product Description

Ribonuclease belongs to pancreatic ribonuclease family. RNase A is an endoribonuclease that attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG.

Application Notes

Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Purification of DNA | Analysis of RNA sequences | RNase protection assays | Hydrolyze RNA contained in protein samples

Specifications
SKU 02193980-CF
Application Notes Ribonuclease A is used to remove RNA from DNA plasmid preparations and protein samples. It is also used in RNA sequence analysis and protection assays.
Biochemical Physiological Actions Ribonuclease A cleaves single stranded RNA after pyrimidine nucleotides.
EC Number 232-646-6
Extinction Coefficient E1%280 = ~7.3
Format Lyophilized powder; Salt-free and protease-free
Grade Molecular Biology Reagent
Hazard Statements H317
Isoelectric Point 9.45
Applications Purification of DNA | Analysis of RNA sequences | RNase protection assays | Hydrolyze RNA contained in protein samples
Personal Protective Equipment Eyeshields, Gloves, respirator filter
pH Optimum pH: 7.0 - 7.5
Physical Appearance White Powder
Preparation Method Chromatographically Purified
RTECS Number RF0760000
Solubility Dissolves readily at 2 mg/mL in analytical grade water to give a clear colorless solution.
Source Bovine Pancrease
Specific Activity ≥70 Kunitz units/mg
Specificity The enzyme is specific for pyrimidine nucleoside linkages; i.e.; those cleaving cyclic 2';3'-pyrimidine nucleotide residues either singly or at the termination of purine nucleotide chains.
Storage and Handling -20 °C; desiccate
Unit Definition One unit causes the hydrolysis of RNA at a rate such that the velocity constant (k) equals 1 at 25 °C at pH 5.0.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.