Chloroform

Chloroform

Synonyms Formyl trichloride; Methane trichloride; Methenyl trichloride; NCI-C02686; RCRA Waste Number U044; TCM; Trichloroform; Trichloromethane

CAS Number: 67-66-3 Molecular Formula: CHCl3 Molecular Weight: 119.369 g/mol

Beilstein Registry Number: 1731042 EC Number: 200-663-8 MDL Number: MFCD00000826

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SKU 02193814-CF
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Description

Product Description

Chloroform

Application Notes

Chloroform is typically used as a solvent and as a cleansing agent. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Used for PCR aqueous phase recovery overlaid with mineral oil||Solvent||Cleansing agent||Chromatography

Specifications
SKU 02193814-CF
Alternate Names Formyl trichloride; Methane trichloride; Methenyl trichloride; NCI-C02686; RCRA Waste Number U044; TCM; Trichloroform; Trichloromethane
Application Notes Chloroform is typically used as a solvent and as a cleansing agent. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.
Auto Ignition Not flammable (USCG, 1999)
Beilstein Registry Number 1731042
Boiling Point 143° F at 760 mm Hg (EPA, 1998)
Density 1.4832 at 68° F (EPA, 1998)
EC Number 200-663-8
Flash Point None (EPA, 1998)
Format liquid
Grade ACS
Hazard Statements H302-H315-H351-H373
Applications Used for PCR aqueous phase recovery overlaid with mineral oil||Solvent||Cleansing agent||Chromatography
MDL # MFCD00000826
Melting Point -82.3° F (EPA, 1998)
Molecular Formula CHCl3
Molecular Weight 119.369 g/mol
Personal Protective Equipment Eyeshields, Faceshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge
Physical Appearance Liquid
Product Families Description Chloroform
Product Overview Chloroform
Refractive Index n20/D 1.445(lit.)
RTECS Number FS9100000
Safety Symbol GHS07, GHS08
Solubility less than 1 mg/mL at 66° F (NTP, 1992)
Storage and Handling Store at Room Temperature (15-30°C). Protect from light.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance λ: 245 nm Amax: 1.00, λ: 255 nm Amax: 0.25, λ: 260 nm Amax: 0.15, λ: 270 nm Amax: 0.05, λ: 290-400 nm Amax: 0.01(Lit.)
Vapor Density 4.12 (EPA, 1998) (Relative to Air)
Vapor Pressure 100 mm Hg at 50.72° F (EPA, 1998)