Coomassie® Brilliant Blue R-250

Coomassie® Brilliant Blue R-250

Synonyms Brilliant Blue R; Acid Blue 83; C.I. 42660; Brilliant indocyanin 6B, Coomassie Brilliant Blue R

CAS Number: 6104-59-2 Molecular Formula: C45H44N3NaO7S2 Molecular Weight: 825.971 g/mol

Beilstein Registry Number: 5718025 EC Number: 228-060-5 MDL Number: MFCD00041762

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SKU 02190682-CF
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Description

Product Description

Coomassie® Brilliant Blue R-250

Application Notes

Brilliant Blue R has been used for many years for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. The Brilliant Blue R-Protein complex has an absorption maximum between 560 and 575 nm. (Scanning is usually done at 570 nm.) The staining intensity of proteins may be related to the basicity of the protein involved. Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates. Coomassie Blue R-250 is additionally used in the Bradford Assay to quantify the concentration of protein in a solution, such as cell lysates. A fast acting, sensitive dye which can be used on SDS gels, IEF gels, and standard gels.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Hematology||Staining

Specifications
SKU 02190682-CF
Alternate Names Brilliant Blue R; Acid Blue 83; C.I. 42660; Brilliant indocyanin 6B, Coomassie Brilliant Blue R
Application Notes Brilliant Blue R has been used for many years for staining proteins on polyacrylamide electrophoresis gels. Improvement of sensitivity and background have been accomplished using Brilliant Blue dyes in a colloidal form. The Brilliant Blue R-Protein complex has an absorption maximum between 560 and 575 nm. (Scanning is usually done at 570 nm.) The staining intensity of proteins may be related to the basicity of the protein involved. Other applications for brilliant Blue R include use in detection of lipids in thin layer chromatography, determination of the CMC of detergents, and detection of proteins in solution, as well as on cellulose acetate plates. Coomassie Blue R-250 is additionally used in the Bradford Assay to quantify the concentration of protein in a solution, such as cell lysates. A fast acting, sensitive dye which can be used on SDS gels, IEF gels, and standard gels.
Beilstein Registry Number 5718025
Biochemical Physiological Actions The dye molecule binds to proteins to form a protein-dye complex. This complex stabilizes the negatively charged anionic form of the dye producing the blue color, even under acidic conditions.
Color Index 42660
EC Number 228-060-5
Format powder
Applications Hematology||Staining
MDL # MFCD00041762
Molecular Formula C45H44N3NaO7S2
Molecular Weight 825.971 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance Purple Powder
Product Families Description Coomassie® Brilliant Blue R-250
Product Overview Coomassie® Brilliant Blue R-250
Storage and Handling Store at Room Temperature(15-30 °C).
Typical Working Concentration Prepare in distilled, deionized water: 50% (v/v) methanol, 0.05% (w/v) Coomassie Brilliant Blue R-250, 10% acetic acid, 40% Water. Dissolve the Coomassie Brilliant Blue R in methanol before adding acetic acid and water (filtration is not generally needed). Solution can be stored for 6 months. If precipitate is observed following prolonged storage, filter to obtain a homogeneous solution.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
UV Visible Absorbance 585 ± 5 nm (ethanol)