Alkaline Phosphatase

Alkaline Phosphatase

Synonyms EC 3.1.3.1; Alkaline phosphomonoesterase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); CIAP

CAS Number: 9001-78-9 Molecular Weight: 496.569 g/mol

EC Number: 232-631-4 MDL Number: MFCD00131849

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Description

Product Description

Alkaline Phosphatase

Application Notes

Alkaline phosphatase is used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations. It is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations||Used in immunoblotting; ELISA′s; and other antibody based detection assays

Specifications
SKU 02190680-CF
Alternate Names EC 3.1.3.1; Alkaline phosphomonoesterase; Orthophosphoric-monoester phosphohydrolase (alkaline optimum); CIAP
Application Notes Alkaline phosphatase is used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations. It is used in the laboratory to remove phosphate monoesters which prevents self-ligation. It is also used in the dairy industry to confirm pasturization of milk. Alkaline phosphatse is commonly conjugated to antibodies and used in immunoblotting, ELISA′s, and other antibody based detection assays.
Biochemical Physiological Actions Catalyzes the cleavage of terminal phosphates from nucleic acids. The free 5′-OH can be phosphorylated with polynucleotide kinase and γ32P-ATP to produce γ32P-end-labeled nucleic acids. Linearized cloning vectors can be prevented from recircularizing by dephosphorylation with alkaline phosphatase.
EC Number 232-631-4
Format powder
Isoelectric Point 5.7(Lit.)
Applications Used to remove 5′-phosphate groups from nucleic acid molecules to prevent self-ligation of DNA fragments and vectors during recombinant DNA manipulations||Used in immunoblotting; ELISA′s; and other antibody based detection assays
MDL # MFCD00131849
Molecular Weight 496.569 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Off-white Powder
Product Families Description Alkaline Phosphatase
Product Overview Alkaline Phosphatase
Source Calf Intestine
Specific Activity ≥1.0 u/mg
Storage and Handling Store at -20 °C.
Unit Definition That amount of enzyme causing the hydrolysis of one micromole of p-nitrophenyl phosphate per minute at 25 °C and pH 9.6.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.