Aldehyde dehydrogenase oxidizes a number of aliphatic and aromatic aldehydes. Acetyl-GSH is not hydrolyzed.
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Acting on aldehydes or oxo groups
|Alternate Names||Aldehyde:NAD[P]+ oxidoreductase; ALDH|
|Application Notes||Aldehyde dehydrogenase oxidizes a number of aliphatic and aromatic aldehydes. Acetyl-GSH is not hydrolyzed.|
|Biochemical Physiological Actions||Aldehyde dehydrogenase is a tetramer and has several different isoforms. The enzyme tested in 0.01 M pyrophosphate buffer shows a sharp optimum around pH 9.3 with acetaldehyde as substrate. Potassium ions and cysteine are essential for the enzyme's activity. Rubidium or NH4+ can be substituted for K+, and glutathione for cysteine. Lithium, Na+, and Cs+ inhibit the reaction. Aldehyde dehydrogenase is inhibited by propylurea, crotonaldehyde, n-propyl isocyanate, cyclohexyl isocyanate, 1-n-propyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea, and 1-methyl-1-[(4-chlorophenyl)sulphonyl]-3-n-propylurea. The enzyme may be utilized to quantitate aldehydes present in blood.|
|Extinction Coefficient||9.4 (279 nm)(Lit)|
|Format||Potassium Activated; Freeze dried stabilized powder|
|Applications||Acting on aldehydes or oxo groups|
|Personal Protective Equipment||Eyeshields, Gloves, respirator filter|
|Physical Appearance||Off-white Powder|
|Product Families Description||Aldehyde Dehydrogenase|
|Product Overview||Aldehyde Dehydrogenase|
|Specific Activity||Protein-~5 u/mg, solid-≥1 u/mg solid|
|Storage and Handling||Store at -20 °C; Desiccated.|
|Unit Definition||One unit causes the oxidation of one µ mole of acetaldehyde to acetic acid per minute at pH 8.0 and 25 °C in the presence of potassium ions and thiols.|