CHAPS--N/H

CHAPS--N/H

Synonyms 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate; N,N-Dimethyl-N-(3-sulfopropyl)-3-[[(3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl] amino]-1-propanaminium inner salt

CAS Number: 75621-03-3 Molecular Formula: C32H58N2O7S Molecular Weight: 614.9

MDL Number: MFCD00012116

Configure Your Product:

SKU 02190319-CF
Price:
$51.55
In Stock
Request a quote
Description

Product Description

CHAPS is a nondenaturing zwitterionic detergent for solubilizing membrane proteins and breaking protein-protein interactions. Combines the useful properties of both the sulfobetaine-type and the bile salt detergents.

Application Notes

CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Detergent for solubilizing membrane proteins

Specifications
SKU 02190319-CF
Aggregation Number 4-14 (0-0.1 M Na+)
Application Notes CHAPS is commonly used for protein solubilization in isoelectric focusing and two-dimensional electrophoresis especially for non-denaturing (without urea) isoelectric focusing. CHAPS has been shown to give excellent resolution of some subcellular preparations and plant proteins. Its small micellar molecular weight (6150) and high CMC (6-10 mM) allow it to be removed from samples by dialysis. Can be used as a non-toxic stabilizing agent for growth factors.
Critical Micelle Concentration 6-10 mM
Hazard Statements H315-H319-H335-H360D
Applications Detergent for solubilizing membrane proteins
Melting Point 265°C (Lit.)
Molecular Formula C32H58N2O7S
Molecular Weight 614.9
pH 4.0 - 7.0 (10% aq soln)
Physical Appearance White Powder
Purity ≥98%
Safety Symbol GHS07, GHS08
Solubility Soluble in water (100 mg/mL - clear, colorless to slightly yellow solution).
Storage and Handling Store at Room Temperature.
Typical Working Concentration Concentrations between 1-4% (v/v) are typically used in an isoelectric focusing gel.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.