3,3',5,5'-Tetramethylbenzidine

3,3',5,5'-Tetramethylbenzidine

Synonyms TMB; TMB substrate; BM blue; Sure Blue TMB; TMB Blotting Plus

CAS Number: 54827-17-7 Molecular Formula: C16H20N2 Molecular Weight: 240.35 g/mol

Beilstein Registry Number: 2808541 EC Number: 259-364-6 MDL Number: MFCD00007748

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SKU 02190277-CF
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Description

Product Description

3,3',5,5'-Tetramethylbenzidine

Application Notes

3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Peroxidase substrate suitable for use in ELISA

Specifications
SKU 02190277-CF
Alternate Names TMB; TMB substrate; BM blue; Sure Blue TMB; TMB Blotting Plus
Application Notes 3,3',5,5'-Tetramethylbenzidine is suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases. It is useful in gel staining procedure for low levels of home-associated peroxidases and for enzyme immunoassay of horseradish peroxidase.
Beilstein Registry Number 2808541
EC Number 259-364-6
Format powder
Hazard Statements H315-H319-H335
Applications Peroxidase substrate suitable for use in ELISA
MDL # MFCD00007748
Melting Point 334 to 336° F (NTP, 1992)
Molecular Formula C16H20N2
Molecular Weight 240.35 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance Off-white Powder
Product Families Description 3,3',5,5'-Tetramethylbenzidine
Product Overview 3,3',5,5'-Tetramethylbenzidine
Purity ≥99%
RTECS Number DV2300000
Safety Symbol GHS07
Solubility less than 1 mg/mL at 68° F (NTP, 1992)
Storage and Handling Store at Room Temperature (15-30 °C). Protect from light.
Typical Working Concentration Solutions for HRP substrate: Dissolve 1.5 mg TMB in 0.1 mL of DMSO. Then add 9.9 ml of 0.1 M sodium acetate (pH 6.0). Finally, add hydrogen peroxide to a concentration of 0.01% and filter through a Whatman #1 paper filter. Prepare solutions fresh daily. Discard any remaining solution at the end of the day.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.