Alcohol Oxidase

Alcohol Oxidase

Synonyms Alcohol oxidase: oxygen oxido-reductase; E.C. 1.1.3.13

CAS Number: 9073-63-6 Molecular Weight: 191.315 g/mol

EC Number: 231-791-2 MDL Number: MFCD00130452

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SKU 02190155-CF
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Description

Product Description

Alcohol Oxidase

Application Notes

Alcohol oxidase is used for qualitative and quantitative determination of methanol or ethanol (blood alcohol) by colorimetric procedures. Unlike present ADH based assays for ethanol in biological specimens, no labile cofactors or trapping agents are required and considerably less enzyme is used per assay. Quantitative determination of alcohol using an oxygen electrode (enzyme electrode) is also possible. With or without catalase, alcohol oxidase can be used to remove alcohol or scavenge oxygen. Using dilute methanol, sensitive samples can be sterilized through release of the products formaldehyde and hydrogen peroxide. The enzyme can also be used to generate H2O2 in radio-iodine labelling procedures.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Colorimetric determination of blood alcohol

Specifications
SKU 02190155-CF
Alternate Names Alcohol oxidase: oxygen oxido-reductase; E.C. 1.1.3.13
Application Notes Alcohol oxidase is used for qualitative and quantitative determination of methanol or ethanol (blood alcohol) by colorimetric procedures. Unlike present ADH based assays for ethanol in biological specimens, no labile cofactors or trapping agents are required and considerably less enzyme is used per assay. Quantitative determination of alcohol using an oxygen electrode (enzyme electrode) is also possible. With or without catalase, alcohol oxidase can be used to remove alcohol or scavenge oxygen. Using dilute methanol, sensitive samples can be sterilized through release of the products formaldehyde and hydrogen peroxide. The enzyme can also be used to generate H2O2 in radio-iodine labelling procedures.
Biochemical Physiological Actions Alcohol oxidase catalyzes the oxidation of short-chain, primary, aliphatic alcohols to their respective aldehydes. It has the highest affinity for methanol. Alcohol oxidase is involved in the metabolism of methanol, which is important for yeasts that utilize methanol as their sole energy source. Alcohol oxidase is primarily found in the peroxisome but may be present in the cytoplasm as well.
EC Number 231-791-2
Format Frozen Liquid
Formulation Details Supplied in 30% Sucrose; 0.1 M Potassium Phosphate Buffer at pH 8.0.
Applications Colorimetric determination of blood alcohol
MDL # MFCD00130452
Molecular Weight 191.315 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance Reddish Brown Frozen Liquid
Product Families Description Alcohol Oxidase
Product Overview Alcohol Oxidase
Source Pichea Pastoris
Specific Activity ≥10 u/mg Protein
Specificity The enzyme is specific for short-chain; linear aliphatic alcohols and oxidizes the following substrates with a relative velocity rate: methanol = ethanol > n-propanol > n-butanol. It also catalyzes the oxidation of allyl and propargyl alcohol; methyl and ethyl mercaptan and fromaldehyde at slower rates. Branched chain alcohols; C2-aldehydes or higher; ketones and organic acids are not substrates. However; some of these reagents can give false positive responses due to trace contamination with alcohol. Oxygen is the only known hydrogen acceptor. At air saturation; the apparent KM for alcohol oxidase is 0.7 mM using methanol and 9 mM using ethanol as the substrate. In oxygen saturated solutions the KM values are higher. The turnover number is 20;000 sec-1.
Storage and Handling Store at -20 °C.
Unit Definition One unit will oxidize 1.0 µmole of methanol to formaldehyde per minute at pH 7.5 at 25 °C.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.