Nuclease P1

Nuclease P1

Synonyms Nucleate 5'-nucleotidohydrolase (3'-phosphohydrolase);Penicillium Nuclease P1; EC 3. 1. 30.1; Deoxyribonuclease P1; Endonuclease P1

CAS Number: 54576-84-0

MDL Number: MFCD00131010

Packaging size: 1 vial

SKU 0218055501
Price:
$806.85
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Description

Product Description

Nuclease P1

Application Notes

Nuclease P1 is useful at removing single stranded strands hanging off the end of double stranded DNA and at completely cleaving melted DNA for simple DNA composition analysis.Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. It has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5' or 3' end group labeled RNA. Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Endonuclease

Specifications
SKU 0218055501
Alternate Names Nucleate 5'-nucleotidohydrolase (3'-phosphohydrolase);Penicillium Nuclease P1; EC 3. 1. 30.1; Deoxyribonuclease P1; Endonuclease P1
Application Notes Nuclease P1 is useful at removing single stranded strands hanging off the end of double stranded DNA and at completely cleaving melted DNA for simple DNA composition analysis.Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. It has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5' or 3' end group labeled RNA. Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.
Biochemical Physiological Actions Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0.
Format solid
Applications Endonuclease
MDL # MFCD00131010
Pack Size 1 vial
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White Solid
Product Families Description Nuclease P1
Product Overview Nuclease P1
Source Penicillin citrinum
Specific Activity ~500 units/vial
Storage and Handling +4 °C.
Unit Definition The amount of enzyme that produces 1 µmole of acid-soluble neucleotides from RNA based on E=10,600 for RNA hydrolysates per minute at 37 °C, pH 5.3. One unit of the enzyme liberates ~ 4 mmoles of orthophosphate from 3'-AMP per minute at 37 °C, pH 7.2
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.