Aldolase is used in an hydrazine assay based on Boyer's modification in which 3-phosphoglyceraldehyde reacts with hydrazine to form a hydrazone which absorbs at 240 nm.
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
|Alternate Names||D -Fructose-1,6-bisphosphate-D -glyceraldehyde-3-phosphate lyase; E.C. 22.214.171.124; Fructose-diphosphate Aldolase|
|Application Notes||Aldolase is used in an hydrazine assay based on Boyer's modification in which 3-phosphoglyceraldehyde reacts with hydrazine to form a hydrazone which absorbs at 240 nm.|
|Biochemical Physiological Actions||Aldolase is involved in gluconeogenesis, the Calvin cycle and glycolysis. Aldolase, from rabbit muscle, is a class I aldolase which forms covalent Schiff base intermediates. The active site of aldolase is in the center of the α/β 8 barrel fold.|
|Isoelectric Point||6.1 (0.1 M Phosphate buffer) (Lit.)|
|Personal Protective Equipment||Eyeshields, Gloves, Respirator filter|
|Physical Appearance||Off-White Shredded Solid|
|Product Families Description||Aldolase|
|Specific Activity||≥10 u/mg Protein|
|Storage and Handling||Store at +4 °C. Store dessicated.|
|Unit Definition||One unit is the change in absorbancy of 1.00 per minute at 25 °C, pH 7.5.|
|Usage Statement||Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.|