Brefeldin A

Brefeldin A

Synonyms (-4-Dihydroxy-2-[6-Hydroxy-1-Heptenyl]-4-Cyclopentane Crotonic Acid 8-Lactone; BFA; Ascotoxin; Cyanein; Decumbin

CAS Number: 20350-15-6 Molecular Formula: C16H24O4 Molecular Weight: 280.364 g/mol

Beilstein Registry Number: 25191 MDL Number: MFCD12913297

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SKU 02159027-CF
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Description

Product Description

Brefeldin A

Application Notes

Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. BFA inhibits protein synthesis in cultured cells and inhibits the transport of secretory and lysosomal proteins at concentrations of 1-10 µg/mL. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Brefeldin A blocks protein transportation into post-Golgi compartments.

Specifications
SKU 02159027-CF
Alternate Names (-4-Dihydroxy-2-[6-Hydroxy-1-Heptenyl]-4-Cyclopentane Crotonic Acid 8-Lactone; BFA; Ascotoxin; Cyanein; Decumbin
Application Notes Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. BFA inhibits protein synthesis in cultured cells and inhibits the transport of secretory and lysosomal proteins at concentrations of 1-10 µg/mL. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles.
Beilstein Registry Number 25191
Biochemical Physiological Actions Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells.
Hazard Statements H301
Applications Brefeldin A blocks protein transportation into post-Golgi compartments.
MDL # MFCD12913297
Molecular Formula C16H24O4
Molecular Weight 280.364 g/mol
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance White Powder
Product Families Description Brefeldin A
Product Overview Brefeldin A
Purity ≥98%
RTECS Number GY8410000
Safety Symbol GHS06
Storage and Handling Store at -20 °C.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.