Stem Cell Factor, Human, Recombinant

Stem Cell Factor, Human, Recombinant

Synonyms SCF; c-Kit ligand; Mast Cell Growth Factor, Steel factor

CAS Number: Not applicable

MDL Number: MFCD00165757

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SKU 02158698-CF
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Description

Product Description

Stem Cell Factor, Human, Recombinant

Application Notes

Stem Cell Factor is a peptide growth factor/cytokine with broad activities, especially related to hematopoiesis. Among the many activities of SCF are the ability to act on early hematopoietic progenitor/stem cells and to stimulate the proliferation and survival of mast cells. Also, SCF is one of the most potent stimulators of multilineage progenitors (CFUGEMM) in both human and murine bone marrow cells. SCF acts synergistically with other growth factors, including erythropoietin, G-CSF, M-CSF, GM-CSF, IL-3, and IL-6, to increase the number and size of colonies of hematopoietic progenitors. SCF appears to play an important role in the survival, proliferation, or migration of primordial germ cells and melanoblasts during both development and maturation.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Cell Culture||Growth Factor||Stimulators of multilineage progenitors

Specifications
SKU 02158698-CF
Alternate Names SCF; c-Kit ligand; Mast Cell Growth Factor, Steel factor
Application Notes Stem Cell Factor is a peptide growth factor/cytokine with broad activities, especially related to hematopoiesis. Among the many activities of SCF are the ability to act on early hematopoietic progenitor/stem cells and to stimulate the proliferation and survival of mast cells. Also, SCF is one of the most potent stimulators of multilineage progenitors (CFUGEMM) in both human and murine bone marrow cells. SCF acts synergistically with other growth factors, including erythropoietin, G-CSF, M-CSF, GM-CSF, IL-3, and IL-6, to increase the number and size of colonies of hematopoietic progenitors. SCF appears to play an important role in the survival, proliferation, or migration of primordial germ cells and melanoblasts during both development and maturation.
Biochemical Physiological Actions Stem Cell Factor (SCF), also known as c-Kit ligand (KL), steel factor (SLF) and mast cell growth factor (MGF), is a 30 kDa glycoprotein with broad activities on various tissues, including hematopoietic cells, pigment cells, and primordial germ cells. SCF is secreted by endothelial cells, fibroblasts, and bone marrow stromal cells as a membrane-bound form which may be cleaved to release the soluble form. Both forms are active in promoting colony formation from murine bone marrow cells, but membrane-bound SCF is more effective in promoting hematopoieses in vivo, suggesting a role in cellular interactions between hematopoietic and stromal cells. The soluble is thought to exist in solution as a noncovalently linked dimer. SCF is structurally related to M-CSF (CSF-1) and Flt-3/Flk-2 Ligand (FL) with all three sharing a similar size, existence of transmembrane and soluble forms, four conserved cysteines, and alternative splicing exon locations, but they share little sequence homology. SCF alone is a modest colony stimulating factor. However, in the presence of other cytokines such as EPO, TPO, GM-CSF, G-CSF, M-CSF, IL-3, and IL-7, SCF is a potent costimulant that works synergistically to increase the size of myeloid, erythroid or lymphoid lineage colonies without influencing the lineage differentiation of the progenitors.
Format solid
Formulation Sterile filtered then the protein was lyophilized with no additives.
Applications Cell Culture||Growth Factor||Stimulators of multilineage progenitors
MDL # MFCD00165757
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White Solid
Product Families Description Stem Cell Factor
Product Overview Stem Cell Factor, Human, Recombinant
Purity ≥98%
Source Expressed in E. coli
Specific Activity ≤20 ng/mL (ED50)
Storage and Handling -20°C; desiccate
Typical Working Concentration SCF exerts its biological activity in the concentration range of 0.2 to 20.0 ng/mL.
Unit Definition Amount of SCF needed for the dose dependent stimulation of M-07E cells.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.