Dino

4′,6-Diamidino-2-Phenylindole Dihydrochloride

4′,6-Diamidino-2-Phenylindole Dihydrochloride

Synonyms 2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride; DAPI dihydrochloride

CAS Number: 28718-90-3 Molecular Formula: C16H15N5·2HCl Molecular Weight: 350.247 g/mol

Beilstein Registry Number: 4894417 EC Number: 249-186-7 MDL Number: MFCD00012681

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SKU 02157574-CF
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Description

Product Description

4′,6-Diamidino-2-Phenylindole Dihydrochloride

Application Notes

4',6-Diamidino-2-phenylindole Dihydrochloride is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Photofootprinting of DNA

Specifications
SKU 02157574-CF
Application Notes 4',6-Diamidino-2-phenylindole Dihydrochloride is several times more sensitive than ethidium bromide for staining DNA in agarose gels. It may be used for photofootprinting of DNA, to detect annealed probes in blotting applications by specifically visualizing the double-stranded complex, and to study the changes in DNA and analyze DNA content during apoptosis using flow cytometry. DAPI staining has also been shown to be a sensitive and specific detection method for mycoplasma.
Beilstein Registry Number 4894417
Biochemical Physiological Actions Cell permeable fluorescent minor groove-binding probe for DNA. Binds to the minor groove of double-stranded DNA (preferentially to AT rich DNA), forming a stable complex which fluoresces approximately 20 times greater than DAPI alone.
EC Number 249-186-7
Extinction Coefficient (263 nm): 30 (water)(Lit.)
Format powder
Applications Photofootprinting of DNA
Molecular Formula C16H15N5·2HCl
Molecular Weight 350.247 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance Yellow Powder
Product Families Description 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI)
Product Overview 4′,6-Diamidino-2-Phenylindole Dihydrochloride
Storage and Handling Store at -0 °C; desiccate; store under nitrogen.
Typical Working Concentration For vital staining, 1 ug/mL in cell growth medium was used.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.