Carbonic Anhydrase

Carbonic Anhydrase

Synonyms Carbonate Dehydratase; Carbonate Hydrolyase

CAS Number: 9001-03-0 Molecular Weight: 397.751 g/mol

EC Number: 232-576-6 MDL Number: MFCD00081467

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SKU 02153879-CF
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Description

Product Description

Carbonic Anhydrase

Application Notes

Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Enzymes||Inhibitors||Substrates

Specifications
SKU 02153879-CF
Alternate Names Carbonate Dehydratase; Carbonate Hydrolyase
Application Notes Carbonic anhydrase from bovine erythrocytes (BCA) has been used to study the effect of removal of enzyme-bound metal ion, Zn2+, on aggregation behavior of the enzyme. Removal of metal ion by a chelator such as EDTA enhances the propensity of the enzyme to adopt the molten-globule state. This state of the enzyme is found to bind to the chaperone-like α-crystallin and prevent aggregation. The enzyme from Sigma has been immobilized to electrochemical transducers in order to obtain develop an analytical device for dissolved CO2 measurement. It has also been used for the thermodynamic analysis of conformational changes in BCA.
Biochemical Physiological Actions Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, this enzyme is an important target for inhibitors with clinical applications for various pathologies such as glaucoma, epilepsy and Parkinson’s disease.
EC Number 232-576-6
Format powder
Hazard Statements H317
Applications Enzymes||Inhibitors||Substrates
MDL # MFCD00081467
Molecular Weight 397.751 g/mol
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance Tan Powder
Product Families Description Carbonic Anhydrase
Product Overview Carbonic Anhydrase
Source Bovine Erythrocytes
Specific Activity >2000 u/mg solid
Storage and Handling Store at 2-8 °C.
Unit Definition The Unit is determined by the electrometic method in which the time required (in seconds) for a saturated CO2 solution to lower the pH of 0.02M Tris-HCI buffer from 8.3 to 6.3 at 0 °C is determined. The time without enzyme is recorded at To; with enzyme T. One unit equals 2X (To-T)/T using the electrometric assay.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.