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Acid Phosphatase

Acid Phosphatase

Synonyms Apase ; Orthophosphoric-monoester phosphohydrolase (acid optimum); Phosphatase, Acid from wheat germ

CAS Number: 9001-77-8 Molecular Weight: 114.144 g/mol

EC Number: 232-630-9 MDL Number: MFCD00131847

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SKU 02153876-CF
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Description

Product Description

Acid Phosphatase

Application Notes

Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Acid phosphatase from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid

Specifications
SKU 02153876-CF
Application Notes Acid phosphatase (APase) non-specifically catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate. It is used to study the production, transport, and recycling of phosphate and the metabolic and energy transduction processes of the cell. Acid phosphatase from wheat germ and has been used to determine the effect of phosphatase treatment on 3F3/2 staining.
Biochemical Physiological Actions Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phosphatase originates from the aleurone and scutellar tissues during germination. It hydrolyzes phytins, ATP, protein phosphates and nucleotide phosphates. It has a role in general metabolic reactions. Wheat embryo APase consists of four isoenzymes. The molecular mass of wheat germ APase is 58 kDa (gel filtration), the pH Optimum is 5.7, the pH Range is 4.0–7.0 and the optimal temperature is 45 °C.
EC Number 232-630-9
Format lyophilized powder
Applications Catalyzes the hydrolysis of monoesters and anhydrides of phosphoric acid
Molecular Weight 114.144 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Freeze-dried Powder
Product Families Description Acid Phosphatase
Product Overview Acid Phosphatase
Source Wheat Germ
Storage and Handling -20 °C
Unit Definition One unit will hydrolyze 1.0 µmole of o-carboxyphenyl phosphate per minute at 25 °C, pH 5.0.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.