This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
|Application Notes||This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.|
|Biochemical Physiological Actions||Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the ß-strands and the N termini of the main a-helices. These zinc ions indicate the location of the active site.|
|Personal Protective Equipment||Eyeshields, Gloves, half-mask respirator|
|Physical Appearance||White Powder|
|Product Families Description||Creatinine Amidohydrolase|
|Product Overview||Creatinine Amidohydrolase|
|Specific Activity||150 units/mg solid|
|Storage and Handling||-20°C=|
|Unit Definition||One unit causes the formation of one µmole of creatinine-picrate per minute at pH 7.5 at 37°C.|
|Usage Statement||Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.|