Creatinine Amidohydrolase

Creatinine Amidohydrolase

CAS Number: 9025-13-2

EC Number: 232-786-8

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SKU 02153491-CF
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Description

Product Description

Creatinine Amidohydrolase

Application Notes

This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Specifications
SKU 02153491-CF
Application Notes This enzyme is useful for enzymatic determination of creatinine when coupled with creatine amidinohydrolase, sarcosine dehydrogenase or sarcosine oxidase and formaldehyde dehydrogenase in clinical analysis.
Biochemical Physiological Actions Creatininase from Pseudomonas sp. is a homohexameric enzyme with a molecular mass of 28.4 kDa per subunit. It is a cyclic amidohydrolase catalysing the reversible conversion of creatinine to creatine. Each monomer contains a binuclear zinc centre near the C termini of the ß-strands and the N termini of the main a-helices. These zinc ions indicate the location of the active site.
EC Number 232-786-8
Format powder
Isoelectric Point 4.7
Personal Protective Equipment Eyeshields, Gloves, half-mask respirator
Physical Appearance White Powder
Product Families Description Creatinine Amidohydrolase
Product Overview Creatinine Amidohydrolase
Source Pseudomonas sp.
Specific Activity 150 units/mg solid
Storage and Handling -20°C=
Unit Definition One unit causes the formation of one µmole of creatinine-picrate per minute at pH 7.5 at 37°C.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.