Dino

Concanavalin A, FITC Conjugated

Concanavalin A, FITC Conjugated

Synonyms Con A

CAS Number: Not applicable

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SKU 02153245-CF
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Description

Product Description

Concanavalin A, Fitc Conjugated

Application Notes

Concanavalin A used to examine tissue sections. It is conjugated to FITC, allowing fluorescent detection in binding assays for example detection of glycoproteins containing α−D-mannose or α−D-glucose. It is also used in blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, ymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Fluorescent detection in binding assays

Specifications
SKU 02153245-CF
Application Notes Concanavalin A used to examine tissue sections. It is conjugated to FITC, allowing fluorescent detection in binding assays for example detection of glycoproteins containing α−D-mannose or α−D-glucose. It is also used in blood grouping and erythrocyte polyagglutination studies, mitogenic stimulation of lymphocytes, ymphocyte subpopulation studies, fractionation of cells and other particles, histochemical studies of normal and pathological conditions.
Biochemical Physiological Actions Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Conjugate FITC conjugate
Format Frozen Solution
Hazard Statements H317
Applications Fluorescent detection in binding assays
Personal Protective Equipment Dust mask, Eyeshields, Faceshields, Gloves
Physical Appearance Frozen Solution
Preparation Method Gel filtration performed after conjugation to remove free FITC. Buffer: 0.05 M Tris- 0.15 M NaCl-0.004 M CaCl2, pH 7.0-7.2. contains sodium azide as a preservative.
Product Families Description Concanavalin A
Product Overview Concanavalin A, Fitc Conjugated
Safety Symbol GHS07
Source Jackbean
Specificity (Carbohydrate) α-D-Mannose; α-D-Glucose; Branched mannose
Storage and Handling Store at -0 °C; protect from light.
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.