E-64

E-64

Synonyms [L-3-trans-Carboxyoxiran-2-carbonyl]-L-leucyl-agmatin; trans-Epoxysuccinyl-L-leucylamido (4-guanidino) butane; N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide; L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatine

CAS Number: 66701-25-5 Molecular Formula: C15H27N5O5 Molecular Weight: 357.411 g/mol

Beilstein Registry Number: 1405664 MDL Number: MFCD00080261

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SKU 02152846-CF
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Description

Product Description

E-64

Application Notes

E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained.

Usage Statement

Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Key Applications

Inhibitor for thiol protease

Specifications
SKU 02152846-CF
Alternate Names [L-3-trans-Carboxyoxiran-2-carbonyl]-L-leucyl-agmatin; trans-Epoxysuccinyl-L-leucylamido (4-guanidino) butane; N-(trans-Epoxysuccinyl)-L-leucine 4-guanidinobutylamide; L-trans-3-Carboxyoxiran-2-carbonyl-L-leucylagmatine
Application Notes E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but specificity is retained.
Beilstein Registry Number 1405664
Biochemical Physiological Actions E-64 is an irreversible, potent, and highly selective cysteine protease inhibitor. E-64 does not react with the functional thiol group of non-protease enzymes, such as L-lactate dehydrogenase or creatine kinase. E-64 will not inhibit serine proteases (except trypsin) like other cysteine protease inhibitors, leupeptin and antipain. The trans-epoxysuccinyl group (active moiety) of E-64 irreversibly binds to an active thiol group in many cysteine proteases, such as papain, actinidase, and cathepsins B, H, and L to form a thioether linkage. E-64 is a very useful cysteine protease inhibitor for use in in vivo studies because it has a specific inhibition, it is permeable in cells and tissues and has low toxicity.
Format powder
Applications Inhibitor for thiol protease
MDL # MFCD00080261
Molecular Formula C15H27N5O5
Molecular Weight 357.411 g/mol
Optical Rotation (c=0.5, 0.1 N HCl): +24.9 ° (Lit.)
Personal Protective Equipment Eyeshields, Gloves, Respirator filter
Physical Appearance White Powder
Product Families Description E-64
Product Overview E-64
RTECS Number RR0390000
Source Synthetic
Storage and Handling Store at -20 °C; protect from light. Store Dessicated.
Typical Working Concentration 1-10 uM
Usage Statement Research Use Only (RUO). Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.