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Beta-Glucuronidase

Beta-Glucuronidase

Synonyms β-D-Glucuronide glucuronosohydrolase

CAS Number: 9001-45-0

EC Number: 232-606-8 MDL Number: MFCD00130629

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SKU 02152284-CF
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Description

Product Description

Beta-glucuronidases are members of the glycosidase family of enzymes that catalyze breakdown of complex carbohydrates. Human β-glucuronidase is a type of glucuronidase that catalyzes hydrolysis of β-D-glucuronic acid residues from the non-reducing end of mucopolysaccharides such as heparan sulfate. In human gut β-glucuronidase converts conjugated bilirubin to the unconjugated form for reabsorption.

Application Notes

β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means.β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Used as reporter gene | Enzymatic hydrolysis of glucuronides

Specifications
SKU 02152284-CF
Application Notes β-Glucuronidases are routinely used for the enzymatic hydrolysis of glucuronides from Serum plasma and other fluids prior to analysis by enzyme immunoassay, mass spectrometry, gas chromatography, high performance liquid chromatography, or other means.β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.
Biochemical Physiological Actions β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.β-Glucuronidase from Helix pomatia is a mixture of enzymes. Inhibitors: D-glucuronic acid, D-galacturonic acid, D-glucaro-1, 4-lactone
EC Number 232-606-8
Format Supplied in 65% Saturated Ammonium Sulfate Solution.
Hazard Statements H317-H334
Applications Used as reporter gene | Enzymatic hydrolysis of glucuronides
Personal Protective Equipment Breathing apparatus,gloves,Safety glasses
Physical Appearance Brown Solution
RTECS Number LZ8990000
Safety Symbol GHS08
Source Helix pomatia
Specific Activity Sulfatase Activity
Sterility ≤5000 u/ml
Storage and Handling +4°C; protect from light; do not freeze
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
UV Visible Absorbance Unit definition: amount that liberates 1.0 µg of phenolphthalein from phenolphthalein glucuronide per hour at pH 5.0 and 37°C.