Pyruvate kinase is often used as a coupling enzyme in conjunction with lactic dehydrogenase in quantifying ADP and the activity of enzymes that catalyze the formation of ADP.
Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
|Analysis Note||Protein determined by biuret.|
|Application Notes||Pyruvate kinase is often used as a coupling enzyme in conjunction with lactic dehydrogenase in quantifying ADP and the activity of enzymes that catalyze the formation of ADP.|
|Biochemical Physiological Actions||Pyruvate kinase is highly specific for phosphoenolpyruvate, but can utilize other dinucleotide triphosphates as substrates in place of ATP including GTP, ITP, dATP, UTP, and CTP.|
|Extinction Coefficient||5.4 (280 nm) (Lit.)|
|Personal Protective Equipment||Eyeshields, Gloves, Repirator Filter|
|Physical Appearance||White Crystalline Powder|
|Product Families Description||Pyruvate Kinase|
|Product Overview||Pyruvate Kinase|
|Specific Activity||≥200 u/mg protein|
|Storage and Handling||Store at -20 °C; desiccate.|
|Unit Definition||That amount of enzyme causing the oxidation of one micromole of NADH per minute at 25 °C and pH 7.4.|
|Usage Statement||Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.|