Protocatechuate-3,4-Dioxygenase

Protocatechuate-3,4-Dioxygenase

CAS Number: 9029-47-4

MDL Number: MFCD00132132

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SKU 02151975-CF
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Description

Product Description

Protocatechuate-3,4-Dioxygenase

Application Notes

Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into ß-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Used for enzymatic determination of choline esterase

Specifications
SKU 02151975-CF
Application Notes Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into ß-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.
Format powder
Applications Used for enzymatic determination of choline esterase
MDL # MFCD00132132
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance Off-white Powder
Product Families Description Protocatechuate-3,4-dioxygenase
Product Overview Protocatechuate-3,4-Dioxygenase
Source Pseudomonas sp.
Specific Activity ≥3 u/mg solid
Storage and Handling Store at -20 °C.
Unit Definition One unit will oxidize one µmole of protocatechuate to 3-carboxy-cis,cis-muconate per minute at pH 7.5 at 37 °C.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.