P-Nitrophenyl Phosphate Di(Tris) Salt

P-Nitrophenyl Phosphate Di(Tris) Salt

Synonyms 4-Nitrophenyl phosphate di(tris) salt; 4-Nitrophenyl phosphate bis(tris) salt; pNPP

CAS Number: 68189-42-4 Molecular Formula: C14H28N3O12P Molecular Weight: 461.361 g/mol

Beilstein Registry Number: 1578142 EC Number: 269-198-6 MDL Number: MFCD00037009

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SKU 02151766-CF
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Description

Product Description

P-Nitrophenyl Phosphate Di(Tris) Salt

Application Notes

p-Nitrophenylphosphate Di(tris) Salt(pNPP) is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.

Usage Statement

Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.

Key Applications

Substrate for assay of alkaline phosphatase

Specifications
SKU 02151766-CF
Application Notes p-Nitrophenylphosphate Di(tris) Salt(pNPP) is the substrate of choice for use with alkaline phosphatase in ELISA procedures. This substrate produces a soluble end product that is yellow in color and can be read spectrophotometrically at 405 nm. The pNPP reaction may be stopped with 3 M NaOH and read at 405 nm.
Beilstein Registry Number 1578142
EC Number 269-198-6
Format powder
Applications Substrate for assay of alkaline phosphatase
Molecular Formula C14H28N3O12P
Molecular Weight 461.361 g/mol
Personal Protective Equipment Eyeshields, Gloves, respirator filter
Physical Appearance White Powder
Product Families Description p-Nitrophenyl phosphate
Product Overview P-Nitrophenyl Phosphate Di(Tris) Salt
Storage and Handling Store at +4 °C.
Usage Statement Research Use Only (RUO). Ready for CE IVD labeling of clinical applications.
UV Visible Absorbance λ max (water) 219-223, 307-311 nm